吲哚菁绿光热治疗耐甲氧西林葡萄球菌感染的研究  

Experimental study of indocyanine green photothermal in the treatment of methicillin-resistantStaphylococcus Aureus infection

作　　者：赵妍[1] 赵晶晶 康莉[2] 李真玉[3] 刘双庆[4] 陈兵[3] ZHAO Yan;ZHAO Jingjing;KANG Li;LI Zhenyu;LIU Shuangqing;CHEN Bing(Department of Endocrinology,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Nephrology,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Critical Care Medicine,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Clinical Laboratory,the Second Hospital of Tianjin Medical University,Tianjin 300211,China)

机构地区：[1]天津医科大学第二医院内分泌科,300211 [2]天津医科大学第二医院肾内科,300211 [3]天津医科大学第二医院重症医学科,300211 [4]天津医科大学第二医院检验科,300211

出　　处：《天津医药》2022年第5期466-470,共5页Tianjin Medical Journal

基　　金：天津市教委高等学校基本科研业务资助项目(2019KJ164)。

摘　　要：目的探讨吲哚菁绿(ICG)的光热治疗(PTT)性能及对耐甲氧西林葡萄球菌的杀灭效果。方法利用紫外和荧光光谱对ICG的光物理性质进行表征;测定不同质量浓度ICG(0、12.5、25、50、100和200 mg/L)的光热升温曲线,评估ICG的光热转化性能;取小鼠乳腺癌4T1细胞与不同质量浓度的ICG溶液(0、50、100、200和500 mg/L)混合,通过MTT实验检测ICG的细胞毒性;取1×1010CFU/mL的耐甲氧西林的金黄色葡萄球菌加入到96孔板中,将实验分为7组:PBS组、ICG组(500 mg/L)、激光照射组(1.5 W/cm^(2)的808 nm激光照射10 min)、ICG+0.33 W/cm^(2)激光照射组、ICG+0.66 W/cm^(2)激光照射组、ICG+1 W/cm^(2)激光照射组、ICG+1.5 W/cm^(2)激光照射组,观察并记录存活的菌落数,分析基于ICG的PTT杀菌效果。结果紫外吸收光谱证实ICG在808 nm处具有较强的光吸收能力;ICG溶液(质量浓度为200 mg/L)经功率密度为1.5 W/cm^(2)的808 nm近红外激光照射10 min后,温度可升高23.3℃,而纯水的温度仅升高了4.8℃。ICG不同质量浓度组即50、100、200、500 mg/L组的细胞生存率分别为81.87%±2.69%、81.47%±2.94%、82.92%±2.61%、80.44%±2.98%,组间差异无统计学意义(F=0.402,P>0.05)。不同功率密度激光照射组即0.33、0.66、1、1.5 W/cm^(2)组的细胞生存率分别为97.21%±1.59%、94.92%±1.98%、93.89%±1.38%、93.03%±2.31%,组间差异无统计学意义(F=2.864,P>0.05)。杀菌实验中,与PBS组比较,ICG+激光照射组随着激光功率密度的升高,细菌存活数量逐渐下降,差异有统计学意义(F=133.800,P<0.01)。结论ICG具有良好的生物相容性和光热治疗性能,在近红外激光照射下对耐甲氧西林葡萄球菌具有较强的杀灭效果,具有较好的临床转化应用价值。Objective To investigate the sterilizing effect of photothermal therapy(PTT)based on indocyanine green(ICG)on methicillin-resistant Staphylococcus Aureus.Methods Ultraviolet and fluorescence spectra were used to characterize the photophysical property of ICG.The photothermal heating curves of ICG with different concentrations(0,12.5,25,50,100 and 200 mg/L)were determined to evaluate the photothermal conversion performance of ICG.Mouse breast cancer 4T1 cells were mixed with different concentrations of ICG solution(0,50,100,200 and 500 mg/L),and the cytotoxicity of ICG was measured by MTT assay.The concentration of methicillin-resistant Staphylococcus Aureus was 1×1010 CFU/mL,and the experiment was divided into the 7 groups:the PBS group,the ICG group(500 mg/L),the laser irradiation group(808 nm laser irradiation of 1.5 W/cm^(2) for 10 min),the ICG+0.33 W/cm^(2) laser irradiation group,the ICG+0.66 W/cm^(2) laser irradiation group,the ICG+1 W/cm^(2) laser irradiation group and the ICG+1.5 W/cm^(2) laser irradiation group.The number of surviving colonies was calculated and the sterilizing effect of ICG-based PTT was analyzed.Results The ultraviolet absorption curve showed that ICG had strong absorption band at 808 nm.The temperature of ICG solution(200 mg/L)can be increased by 23.3℃after irradiation by 808 nm near-infrared laser with 1.5 W/cm^(2) for 10 min,while the temperature of pure water only increased by only 4.8℃at the same experimental condition.The relative survival rates of ICG groups with different concentrations(50 mg/L,100 mg/L,200 mg/L and 500 mg/L)were 81.87%±2.69%,81.47%±2.94%,82.92%±2.61%and 80.44%±2.98%,respectively.There were no significant differences between the groups(F=0.402,P>0.05).The survival rates of different laser irradiation groups(0.33,0.66,1 and 1.5 W/cm^(2))were 94.92%±1.98%,93.89%±1.38%and 93.03%±2.31%respectively,and there was no significant difference between the groups(F=2.864,P>0.05).In the sterilization experiment,compared with the PBS group,the number of surviving

关 键 词：激光 耐甲氧西林金黄色葡萄球菌 材料试验 光热治疗 吲哚菁绿 杀菌 

分 类 号：R378.11[医药卫生—病原生物学]