蛋白质磷酸酶2A激活剂对星形胶质细胞迁移能力的影响及作用机制研究  

Effects of PP2A activators on the migration ability of astrocytes and its mechanism

作　　者：张黎军[1] 赵盼盼[1] 徐志秀 王凡 王朔 任静 袁彬[1] 吉四辈[1] ZHANG Lijun;ZHAO Panpan;XU Zhixiu;WANG Fan;WANG Shuo;REN Jing;YUAN Bin;JI Sibei(Department of Neurology,the First Affiliated Hospital of Xinxiang Medical College,Xinxiang 453100,China)

机构地区：[1]新乡医学院第一附属医院神经内科二病区,453100

出　　处：《浙江医学》2022年第8期818-821,共4页Zhejiang Medical Journal

基　　金：河南省医学科技攻关计划项目(2018020368)。

摘　　要：目的探讨蛋白质磷酸酶2A(PP2A)激活剂对星形胶质细胞迁移能力的影响及相关作用机制。方法选取出生48 h内健康、清洁级SD雄性大鼠,分离星形胶质细胞,分为抑制组(加入15 nM PP2A抑制剂冈田酸)、激活组(加入15 nM PP2A激活剂鞘胺醇)、对照组(加入等体积二甲基亚砜)。采用PP2A活性检测试剂盒检测PP2A活性,检测细胞迁移率,并采用Western blot法检测磷酸化p38(p-p38)、基质金属蛋白酶(MMP)-2、MMP-9表达。结果抑制组PP2A活性(51.22±8.98)均低于激活组(135.89±11.25)及对照组(102.15±10.15),对照组PP2A活性低于激活组,差异均有统计学意义(均P<0.05)。抑制组MMP-2和MMP-9蛋白表达均低于对照组和激活组,p-p38蛋白表达高于对照组和激活组,对照组MMP-2和MMP-9蛋白表达均低于激活组,p-p38蛋白表达高于激活组,差异均有统计学意义(均P<0.05)。结论PP2A激活剂通过调控p38MAPK信号通路来提高星形胶质细胞中PP2A活性及星形胶质细胞迁移能力。Objective To investigate the effect of protein phosphatase 2A(PP2A)activator on the migration ability of astrocytes and its mechanism.Methods Astrocytes were isolated from healthy young male SD rats born within 48 h.The cultured rat astrocytes were treated with DMSO(control group),15nm PP2A inhibitor OA(inhibition group)or 15nm PP2A activator DES(activation group).PP2A activity detection kit was used to detect PP2A activity,Transwell was used to detect cell mobility,and Western blot was used to detect the protein expression of phosphorylated p38(p-p38),matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9).Results The activity of PP2A in the inhibition group(51.22±8.98)was significantly lower than that in the control group(102.15±10.15)and the activation group(135.89±11.25)(P<0.05),while the activity of PP2A in the control group was lower than that in the activation group(P<0.05).The cell migration rate of inhibition group was lower than that of control group and activation group,while the cell migration rate in the control group was significantly lower than that in the activation group(P<0.05).The expression of MMP-2 and MMP-9 protein in inhibition group was lower than that in control group and activation group,and the expression of p-p38 protein was higher than that in control group and activation group,while the expression of MMP-2 and MMP-9 protein in the control group was lower than that in the activation group,and the expression of p-p38 protein was higher than that in the activation group(P<0.05).Conclusion PP2A activator can improve the activity of PP2A and the migration ability of astrocytes by regulating p38MAPK signal pathway.

关 键 词：蛋白质磷酸酶2A 星形胶质细胞 迁移 作用机制 

分 类 号：R741[医药卫生—神经病学与精神病学]