Nerve growth factor-basic fibroblast growth factor poly-lactide co-glycolid sustained-release microspheres and the small gap sleeve bridging technique to repair peripheral nerve injury  

作　　者：Ming Li Ting-Min Xu Dian-Ying Zhang Xiao-Meng Zhang Feng Rao Si-Zheng Zhan Man Ma Chen Xiong Xiao-Feng Chen Yan-Hua Wang 

机构地区：[1]Department of Trauma and Orthopedics,Peking University People’s Hospital,Beijing,China [2]Key Laboratory of Trauma and Neural Regeneration(Peking University),Ministry of Education,Beijing,China [3]Trauma Medicine Center,Peking University People’s Hospital,National Center for Trauma Medicine,Beijing,China

出　　处：《Neural Regeneration Research》2023年第1期162-169,共8页中国神经再生研究（英文版）

基　　金：supported by the National Key Research and Development Program of China, No. 2016YFC1101603 (to DYZ);the National Natural Science Foundation of China, Nos. 31640045 (to YHW), 81901251 (to ML);the Natural Science Foundation of Beijing of China, No. 7204323 (to ML)

摘　　要：We previously prepared nerve growth factor poly-lactide co-glycolid sustained-release microspheres to treat rat sciatic nerve injury using the small gap sleeve technique.Multiple growth factors play a synergistic role in promoting the repair of peripheral nerve injury;as a result,in this study,we added basic fibroblast growth factors to the microspheres to further promote nerve regeneration.First,in an in vitro biomimetic microenvironment,we developed and used a drug screening biomimetic microfluidic chip to screen the optimal combination of nerve growth factor/basic fibroblast growth factor to promote the regeneration of Schwann cells.We found that 22.56 ng/mL nerve growth factor combined with 4.29 ng/mL basic fibroblast growth factor exhibited optimal effects on the proliferation of primary rat Schwann cells.The successfully prepared nerve growth factor-basic fibroblast growth factor-poly-lactide-co-glycolid sustained-release microspheres were used to treat rat sciatic nerve transection injury using the small gap sleeve bridge technique.Compared with epithelium sutures and small gap sleeve bridging alone,the small gap sleeve bridging technique combined with drug-free sustained-release microspheres has a stronger effect on rat sciatic nerve transfection injury repair at the structural and functional level.

关 键 词：biomimetic microfluidic chip growth factor in vitro biomimetic microenvironment nerve function peripheral nerve injury sciatic nerve small gap sleeve bridging sustained-release microspheres 

分 类 号：R741[医药卫生—神经病学与精神病学]