3种副伤寒沙门氏菌的傅里叶变换红外光谱快速分类鉴定  

Rapid Differentiation and Identification of Three Subspecies of Salmonella paratyphi by FT-IR Spectroscopy

作　　者：陈琨 汪颖 王静 龚方 兰冬雪 瞿茜楠 李兆杰 Chen Kun;Wang Ying;Wang Jing;Gong Fang;Lan Dongxue;Qu Xinan;Li Zhaojie(Qingdao Agricultural University,Qingdao 266109,Shandong;Weihai Customs,Weihai 264205,Shandong;The CPC Qingdao West Coast New Area Working Committee Military and Civilian Integration Office,Qingdao 266000,Shandong;Qingdao Special Food Research Institute,Qingdao 266109,Shandong)

机构地区：[1]青岛农业大学,山东青岛266109 [2]威海海关,山东威海264205 [3]中共青岛西海岸新区工委军民融合办,山东青岛266000 [4]青岛特种食品研究院,山东青岛266109

出　　处：《中国食品学报》2022年第4期375-381,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：山东省自然科学基金面上项目(ZR2020MC217);山东省青创团队培育项目(2019-135)。

摘　　要：建立高效的致病菌分类、鉴定方法是保障食品卫生安全和人类健康的重要技术手段。红外光谱技术具有信息全面,分辨率高,鉴定速度快等优点。本文采用傅里叶变换红外光谱技术采集副伤寒沙门氏菌的3个亚种(甲型、乙型、丙型)的近红外光谱,建立这3种菌的一阶导数谱数据库。结合主成分分析和分级聚类分析两种化学计量分析方法,成功建立不同亚种菌种的聚类分析模型,实现副伤寒沙门氏菌亚种水平上的区分;加标验证试验证明建立的标准菌株光谱数据库和聚类分析模型可用于可疑目标菌的分类鉴定。研究结果表明FT-IR技术可用于甲型、乙型、丙型副伤寒沙门氏菌的分类鉴定,具有操作简便、快捷,结果准确,成本低廉等优点。Efficient method for differentiation and identification of pathogenic bacteria was important to ensure food safety and human health. Infrared spectral technology has the advantages of comprehensive information, high resolution, and fast identification. In this study, FT-IR fingerprint absorption spectra of three subspecies of Salmonella paratyphi were collected, and a standard spectral derivatives library was created. Combined with chemometrics methods, two cluster models of PCA and HCA were established. It was found that three subspecies of Salmonella paratyphi could be well differentiated and identified. Spiked assays proved that the standard spectral derivatives library and the cluster models could be used to differentiate and identify the three subspecies of Salmonella paratyphi. As a rapid, easy-to-use, accurate, and low cost technique, FT-IR spectroscopy is an effective tool to differentiate and identify three subspecies of Salmonella paratyphi.

关 键 词：傅里叶变换红外光谱 副伤寒沙门氏菌 亚种 主成分分析 分级聚类分析 分类 鉴定 

分 类 号：O657.33[理学—分析化学] TS201.3[理学—化学]