乳腺癌循环肿瘤细胞磷脂酰肌醇-3激酶p110催化亚单位基因突变与临床病理特征的关系  

Relationship between the Phosphatidylinositol-3 Kinase p110 Catalytic Subunit Gene Mutation of Circulating Tumor Cells and Clinicopathological Characteristics in Breast Cancer Patients

作　　者：王巧鸽 闫丰娜 WANG Qiao-ge;YAN Feng-na(Department of Pathology,Northwest Women and Children's Hospital,Xi'an 710061)

机构地区：[1]西北妇女儿童医院病理科,陕西西安710061 [2]商洛国际医学中心医院病理科,陕西商洛726000

出　　处：《医学临床研究》2022年第4期542-545,共4页Journal of Clinical Research

摘　　要：【目的】探讨乳腺癌循环肿瘤细胞磷脂酰肌醇-3激酶p110催化亚单位(PIK3CA)基因突变与临床病理特征的关系。【方法】选取两院收治的78例乳腺癌患者为观察组,另选取同期来院体检的42例健康女性作为对照组;采用微滴式数字技术检查两组外周血循环肿瘤细胞中PIK3CA基因突变情况,统计乳腺癌患者的临床特征,采用Kaplan-Meier法进行生存分析。【结果】乳腺癌患者外周血循环肿瘤细胞中PIK3CA阳性表达率高于对照组(P<0.05);78例患者外周血循环肿瘤细胞中PIK3CA基因突变率为29.49%(23/78);有PIK3CA基因突变患者的临床分期、分化程度、细胞增殖抗原(Ki-67)指数构成比与未突变患者比较,差异均有统计学意义(P<0.05)。乳腺癌患者3年生存情况显示,PIK3CA基因突变患者生存率为56.52%(13/23),明显低于未突变患者的83.02%(44/53),且二者相比较差异有统计学意义(P<0.05)。【结论】临床分期Ⅲ~Ⅳ期、低分化、Ki-67指数≥20%均与乳腺癌患者循环肿瘤细胞中PIK3CA基因突变有关,PIK3CA基因突变在乳腺癌预后中具有重要价值。【Objective】To explore the relationship of phosphatidylinositol-3 kinase p110 catalytic subunit(PIK3CA)gene mutations in circulating tumor cells with clinicopathological characteristics in breast cancer patients.【Methods】Seventy-eight breast cancer patients admitted to the hospital were selected as the observation group,and 72 healthy women who came to the hospital for physical examination during the same period were selected as the control group.The microdrop digital technology was used to examine the PIK3CA mutations in the peripheral blood circulating tumor cells in the two groups,and the clinical characteristics of breast cancer patients were counted.Kaplan-Meier was used for survival analysis.【Results】The positive expression rate of peripheral blood PIK3CA in peripheral blood circulating tumor cells of breast cancer patients were higher than that in the control group(P<0.05).The PIK3CA gene mutation rate of circulating tumor cells in the peripheral blood of 78 breast cancer patients was 29.49%(23/78).The clinical stage,degree of differentiation,and Ki-67 index composition of patients with PIK3CA gene mutations in peripheral blood circulating tumor cells of breast cancer were significantly different from those withou tmutation patients,the difference was statistically significant(P<0.05).The 3-year survival of breast cancer patients showed that the survival rate of patients with PIK3CA gene mutations in peripheral blood circulating tumor cells was 43.48%(13/23),which was lower than that of non-mutated patients 83.02%(44/53).The difference between the two were statistically significant(P<0.05).【Conclusion】The clinical stagesⅢ-Ⅳ,poor differentiation,and Ki-67 index≥20%are all related to the PIK3CA gene mutation in circulating tumor cells of breast cancer patients.It suggests that PIK3CA gene mutation has important value in the prognosis of breast cancer.

关 键 词：乳腺肿瘤/病理学 肿瘤细胞 循环 基因 

分 类 号：R737.9[医药卫生—肿瘤]