^(125)I粒子平面照射对胃癌干细胞增殖和凋亡影响的体外研究  

Effect of^(125)I on proliferation and apoptosis of gastric cancer stem cells

作　　者：杜玲娟[1] 杨镛[1] 李国剑[1] 万嘉[1] 杨国凯[1] 马振桓[1] DU Ling-juan;YANG Yong;LI Guo-jian(Department of Vascular Surgery Center,Yunnan Second People's Hospital/Affiliated Hospital of Yunnan University,Kunming Yunnan 650021,China)

机构地区：[1]云南省第二人民医院/云南大学附属医院血管外科中心,云南昆明650021

出　　处：《临床和实验医学杂志》2022年第7期686-690,共5页Journal of Clinical and Experimental Medicine

基　　金：云南省腹部微创外科研究中心科研项目(编号:2018NS0008)。

摘　　要：目的通过体外研究探讨^(125)I粒子平面照射对胃癌干细胞增殖与凋亡的影响,为^(125)I粒子用于胃癌的靶向干预提供理论依据。方法免疫磁珠分选法分离人胃癌细胞中的CD44(+)细胞,流式细胞术检测CD44(+)表达率。分组采用100、200、300 cGy剂量^(125)I粒子平面照射胃癌干细胞。3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)实验检测各组细胞的生长曲线。实时荧光定量聚合酶链式反应(RT-qPCR)、蛋白免疫印迹以及免疫荧光染色检测缺氧诱导因子1α(HIF-1α)、BNIP3和WNT9Ad的表达情况。流式细胞法检测各组细胞的凋亡情况。结果免疫磁珠分选后富集了79.7%CD44(+)胃癌细胞,且分选后胃癌干细胞生长状态良好。100、200、300 cGy剂量^(125)I粒子平面照射处理可以明显抑制胃癌干细胞的增殖,且抑制作用呈现剂量依赖性(P<0.05)。100、200、300 cGy剂量^(125)I粒子平面照射处理可以明显促进干细胞的凋亡,且促进作用呈现剂量依赖性(P<0.05)。结论体外研究结果显示,^(125)I粒子平面照射会抑制胃癌干细胞增殖,并促进干细胞凋亡。Objective To investigate the effect of^(125)I seed planar irradiation on the proliferation and apoptosis of gastric cancer stem cells(GCSCs),and to provide the further theoretical foundation for the use of^(125)I for targeted intervention in gastric cancer.Methods CD44(+)cells were separated from human gastric cancer cells by magnetic bead cell sorting,and CD44(+)expression rate was detected by flow cytometry.Groups were irradiated with GCSCs using 100,200,300 cGy doses of^(125)I seed plane irradiation.MTT assay was performed to detect the growth curve of GCSCs in each group.RT-qPCR,Western blotting,and immunofluorescence staining were used to detect the expression of hypoxia-inducible factor 1α(HIF-1),BNIP3,and WNT9A.Apoptosis of GCSCs in each group was detected by flow cytometry.Results Magnetic bead cell sorting enriched 79.7%CD44(+),and the growth status of CD44(+)was good after sorting.The treatment with 100,200,300 cGy doses of^(125)I seed plane irradiation significantly inhibited the proliferation of GCSCs,and the inhibitory effect was dose-dependent(P<0.05).The treatment with 100,200,300 cGy doses of^(125)I seed plane irradiation significantly promoted the apoptosis of GCSCs,and the promotion effect was dose-dependent(P<0.05).Conclusion The results of the in vitro study showed that plane irradiation of^(125)I seed inhibits the proliferation of GCSCs and promotes apoptosis of GCSCs.

关 键 词：胃癌干细胞 细胞增殖 细胞凋亡 ^(125)I粒子 平面照射 体外研究 

分 类 号：R735.2[医药卫生—肿瘤]