Old commercialized magnetic particles new trick: Intrinsic internal standard  

作　　者：Chaoqun Wang Ziqiang Deng Hu Zhang Rui Liu Yi Lv 

机构地区：[1]Analytical&Testing Center,Sichuan University,Chengdu 610064,China [2]Key Laboratory of Green Chemistry and Technology of Ministry of Education,College of Chemistry,Sichuan University,Chengdu 610064,China [3]Department of Gastroenterology,West China Hospital,Sichuan University,Chengdu 610041,China

出　　处：《Chinese Chemical Letters》2022年第3期1267-1270,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos.22074096 and 22074098);Talents Program of Sichuan Province (No.903);1.3.5 Project for Disciplines of Excellence of West China Hospital,Sichuan University (No.ZYJC18037);the Fundamental Research Funds for the Central Universities(No.20826041D4117)。

摘　　要：Magnetic particles (MPs) are the most widely used commercialized engineering particles, which gained great success in various biological applications. Inspired by their intrinsic Fe isotope composition, we discovered a commercialized MPs-internal standard's novel function to realize the accurate quantification of biomolecules. The bioassay of carcinoembryonic antigen (CEA) was chosen as a modal system. The Fe isotope in MPs and Au isotope in report probes were simultaneously and sensitively detected by the elemental mass spectrometry. ^(197)Au/^(57)Fe isotopic ratios and CEA concentrations showed good linearity in the range of 0.6-300 ng/mL, with a detection limit of 0.09 ng/mL (3σ). The accuracy and precision of the proposed MPs-based immunoassay were greatly improved, by eliminating potential MPs loss during magnetic separation and absolute intensity fluctuations. Considering the exceptional availability and universality of commercialized MPs, the proposed method might open a new avenue for MPs' biological applications.

关 键 词：Magnetic particles RATIOMETRIC IMMUNOASSAY ICPMS Carcinoembryonic antigen 

分 类 号：Q503[生物学—生物化学]