草鱼感染GCRV后血液中淋巴细胞变化及BCL10表达分析  

LYMPHOCYTES AND BCL10 EXPRESSION IN GRASS CARP(CTENOPHARYNGODON IDELLA)AFTER THE INFECTION OF GCRV

作　　者：姜祝祥 郭早枣 郑国栋[1,2,3] 邹曙明 JIANG Zhu-Xiang;GUO Zao-Zao;ZHENG Guo-Dong;ZOU Shu-Ming(Genetics and Breeding Center for Blunt Snout Bream,Shanghai 201306;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai 201306;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学农业农村部团头鲂遗传育种中心,上海201306 [2]农业农村部淡水水产种质资源重点实验室,上海201306 [3]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《水生生物学报》2022年第4期514-520,共7页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2020YFD0900400);国家自然科学基金(32002381);中国博士后科学基金(2019M651473)资助。

摘　　要：为了研究草鱼BCL10基因在草鱼出血病中的应答机制,文章克隆了BCL10基因,并利用生物信息学、荧光定量和血涂片等技术对其进行了分析。生物信息学结果显示,BCL10基因开放阅读框为738 bp,编码245个氨基酸。实时荧光定量PCR结果显示,感染病毒后草鱼体内BCL10表达量持续上调,在肝胰腺和中肾中第4天达到峰值,第7天表达量开始下调。血涂片显微镜观察发现了血液中淋巴细胞在感染病毒后第1到第4天下降,第7天时上升。肾脏的组织病理学观察也发现中肾中肾小管上皮细胞第1到第7天逐渐空泡化,脱落坏死。以上结果表明,BCL10基因参与了草鱼应对草鱼呼肠孤病毒(GCRV)入侵的免疫应答。Grass carp(Ctenopharyngodon idella)is an essential freshwater aquaculture species in the world.The pro-duction of grass carp will be affected by grass carp hemorrhagic disease.The pathogen of grass carp hemorrhagic di-sease is grass carp reovirus(GCRV).Innate immune system is considered an essential defense for fish against a broad spectrum of pathogens.After the infection of the virus,the innate immune system can protect the host against the inva-sion.One of the innate immune response cell components is lymphocytes.Lymphocytes exist in abundance in the blood.Another typical immune response is inflammation inside the body.Many inflammation researches focus on the Toll-like receptors(TLRs)and Toll-interacting genes,but BCL10 also seems to be of great importance as the positive regulator.It has the function to activate the NF-kappaB pathway and then induce inflammation response.Besides,BCL10 also can be the termination of immune cell signaling.Our research found that GCRV infection reduced the number of the lymphocytes on day 1 and day 4 and then in-creased it on day 7,indicating the participation of lymphocytes in the fish immune defense.Besides,the vascular de-generation and exfoliation and necrosis of cells in trunk kidney indicated damaged blood circulatory system.Bioin-formatics analysis showed that the open reading frame of the BCL10 was 738 bp,encoding 245 amino acids.Phyloge-netic tree analysis showed that grass carp BCL10 has a good clustering relationship with the orthologs of other bony fish species.With the Real-time qPCR result,we found that after the infection of GCRV,BCL10 expression on the first day(D1)up-regulated in the hepatopancreas and gill compared to the control group.In contrast,blood cells and trunk kidney were significantly up-regulated.On the fourth day(D4),the expression of the BCL10 significantly up-regulated in all organs,but on the 7th day(D7),BCL10 expression was down-regulated in all organs compared to the control group.We speculated that as the immune organs in immune response,bloo

关 键 词：GCRV BCL10 表达分析 血淋巴细胞 组织观察 草鱼 

分 类 号：S941.4[农业科学—水产养殖]