HPLC-DAD法测定肠胃舒胶囊中木香烃内酯、去氢木香内酯、没食子酸和血竭素  

Determination of aucklan lactone,dehydrocarbactone,gallic acid,and draconisin in Changweishu Capsules by HPLC-DAD

作　　者：武蕊 崔艳华 黄牧坤 李明杰 WU Rui;CUI Yan-hua;HUANG Mu-kun;LI Ming-jie(Department of Pharmacy,3201 Hospital,Hanzhong 723000,China)

机构地区：[1]三二〇一医院药学部,陕西汉中723000

出　　处：《现代药物与临床》2022年第4期746-749,共4页Drugs & Clinic

摘　　要：目的采用高效液相色谱–二极管阵列检测器(HPLC-DAD)法测定肠胃舒胶囊中木香烃内酯、去氢木香内酯、没食子酸和血竭素。方法采用Waters Sunfire C18色谱柱(250 mm×4.6 mm,5μm);DAD;流动相:乙腈–0.2%磷酸溶液,梯度洗脱;检测波长:木香烃内酯(225 nm)、去氢木香内酯(225 nm)、没食子酸(272 nm)、血竭素(440 nm);体积流量1.0 mL/min;柱温25℃;进样体积10μL。结果木香烃内酯、去氢木香内酯、没食子酸、血竭素分别在0.999~99.900、0.995~99.500、0.275~27.500、0.250~25.000μg/mL线性良好,平均回收率分别为99.2%、99.5%、99.1%、99.9%,RSD值分别为1.1%、1.6%、1.3%、1.7%。结论本法具有简便、准确等特点,可以用于肠胃舒胶囊的质量控制。Objective To establish an HPLC-DAD method for determination of aucklan lactone,dehydrocarbactone,gallic acid,and draconisin in Changweishu Capsules.Methods Waters Sunfire C18 column(250 mm×4.6 mm,5μm)was used with acetonitrile-0.2%phosphoric acid solution as the mobile phase by gradient elution.DAD detector was used for quantitative detection.The detection wavelength were 225 nm(aucklan lactone),225 nm(dehydrocarbactone),272 nm(gallic acid),and 440 nm(draconis).The flow rate was 1.0 mL/min,the column temperature was 25℃,and the injection volume was 10μL.Results The linearity of lignan lactone,dehydrocarbactone,gallic acid and draconisin were good in the range of 0.999—99.900μg/mL,0.995—99.500μg/mL,0.275—27.500μg/mL,and 0.250—25.000μg/mL.The average recoveries were 99.2%,99.5%,99.1%and 99.9%,with RSD 2.4%,1.6%,2.1%,and 1.8%,respectively..Conclusion The method is simple and accurate,which can be used for the quality control of Changweishu Capsules.

关 键 词：肠胃舒胶囊 木香烃内酯 去氢木香内酯 没食子酸 血竭素 HPLC-DAD 

分 类 号：R286.02[医药卫生—中药学]