过表达FGFRL1抑制HCT116人结肠癌细胞的增殖和迁移并促进其凋亡  

Overexpression of fibroblast growth factor receptor like 1(FGFRL1)inhibits proliferation and migration of HCT116 human colon cancer cells,and promotes their apoptosis

作　　者：关丽娜[1] 董瑶 陈思敏[1] 王朝莉[2] 冯莉[2] 谢勇恩[2] 唐学贵[1] GUAN Lina;DONG Yao;CHEN Simin;WANG Chaoli;FENG Li;XIE Yongen;TANG Xuegui(Institute of Anorectal Disease,North Sichuan Medical College,Nanchong 637007,China;Institute of Immunology and Molecular Biology,North Sichuan Medical College,Nanchong 637007,China)

机构地区：[1]川北医学院肛肠疾病研究所,四川南充637007 [2]川北医学院免疫学与分子生物学研究所,四川南充637007

出　　处：《细胞与分子免疫学杂志》2022年第3期251-256,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81573990)。

摘　　要：目的探讨成纤维细胞生长因子受体样蛋白1(FGFRL1)过表达对HCT116结肠癌细胞生物学行为的影响。方法构建可在哺乳动物细胞内表达FGFRL1的重组质粒pcDNA3.1-FGFRL1,转染结肠癌HCT116细胞后,通过持续的G418筛选获取稳定表达细胞系,实时定量PCR和Western blot法检测HCT116细胞FGFRL1的表达。随后将细胞分成空白组(未经处理的HCT116细胞)、阴性对照组(稳定转染空载体的HCT116细胞)及实验组(稳定转染pcDNA3.1-FGFRL1的HCT116细胞)。采用CCK-8试剂盒检测细胞增殖能力,Transwell^(TM)小室实验检测细胞迁移能力,流式细胞术检测细胞凋亡。结果FGFRL1过表达组FGFRL1 mRNA和蛋白水平显著升高。过表达FGFRL1后,HCT116细胞增殖明显降低、细胞迁移能力明显下降、细胞凋亡明显增加。结论过表达FGFRL1抑制HCT116结肠癌细胞的增殖和迁移,并促进其凋亡。Objective To investigate the effect of fibroblast growth factor receptor like 1(FGFRL1) overexpression on the biological behavior of HCT116 human colon cancer cell line. Methods A recombinant plasmid, named as pcDNA3.1-FGFRL1 which expresses FGFRL1 in mammal cells, was constructed. After a transfection of HCT116 cells with pcDNA3.1-FGFRL1, the stable expression cell line was obtained via continual selection with G418, and FGFRL1 expression was analyzed by real time quantitative PCR and Western blotting. In the following experiment, cells were divided into three groups: the blank group(untreated HCT116 cells), the negative group(empty vector stably transfected cells) and the experience group(pcDNA3.1-FGFRL1 stably transfected cells). Cell proliferation was detected by CCK-8 assay. Cell migration ability was analyzed with Transwell^(TM) assay and their apoptosis was evaluated by flow cytometry. Results FGFRL1 mRNA and protein levels increased significantly in FGFRL1 overexpression group. After the overexpression of FGFRL1, proliferation and migration of HCT116 cells dropped significantly, while their apoptosis increased significantly. ConclusionOverexpression of FGFRL1 inhibits the proliferation and migration of colon cancer HCT116 cells and promotes their apoptosis.

关 键 词：成纤维细胞生长因子受体样蛋白1(FGFRL1) 结肠癌细胞 细胞增殖 细胞凋亡 细胞迁移 

分 类 号：Q279[生物学—细胞生物学] R392-33[医药卫生—免疫学]