海洋真菌Penicillium sp.产抗肿瘤天然产物brefeldin A的发酵优化研究  

Research on the fermentation optimization of the marine-derived Penicillium sp. fungus to produce the antitumor natural product brefeldin A

作　　者：武艳伟 张群 滕云飞 姜瑶瑶 邵长伦[1] 魏美燕[1,2] WU Yan-wei;ZHANG Qun;TENG Yun-fei;JIANG Yao-yao;SHAO Chang-lun;WEI Mei-yan(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国海洋药物》2022年第2期14-18,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(U1706210,41776141,41606172)资助。

摘　　要：目的 对海洋来源真菌Penicillium sp.产抗肿瘤天然产物brefeldin A进行发酵优化,以期获得高产率目标化合物。方法 运用单因素实验设计,重点考察了培养方式、发酵培养基组成(如碳源、氮源、盐度等)以及培养条件(温度、摇床转速)等因素。结果 最佳发酵条件为:马铃薯200 g/L,麦芽糖10 g/L,淀粉20 g/L,海盐15 g/L,28℃,摇床转速160 r/min,培养14 d。结论 在该发酵条件下,brefeldin A的实际产量达到40 mg/L,较优化前提高了近10倍。Objective To optimize the fermentation conditions of the marine-derived Penicillium sp. fungus producing the antitumor natural product brefeldin A with high yields. Methods Using single-factor experiments, the influence factors of the culture way, fermentation medium compositions(carbon source, nitrogen source, salinity, etc.) and culture conditions(temperature, shaker speed) were studied. Results The optimal fermentation conditions were as follows: potato 200 g/L, maltose 10 g/L, starch 20 g/L, sea salt 15 g/L, shaking speed 160 r/min at 28 ℃ for 14 days. Conclusion Under the optimal fermentation conditions, the actual yield of brefeldin A was 40 mg/L, which was nearly 10-fold increased than the original condition.

关 键 词：海洋来源真菌 brefeldin A 发酵条件 优化 

分 类 号：R931[医药卫生—生药学]