5次跨膜蛋白及POU3同源盒基因3相关长链非编码RNA在乳腺癌中的表达及其与细胞体外增殖和侵袭能力的关系  

Expression of 5 transmembrane proteins and POU3 homobox gene 3 related long non-coding RNAs in breast cancer and their relationship with cell proliferation and invasion in vitro

作　　者：王钢乐[1] 董懿[1] 曹技磊 纪楠[1] 刘爱蕙[1] 刘恒[1] 任宇[1] Wang Gangle;Dong Yi;Cao Jilei;Ji Nan;Liu Aihui;Liu Heng;Ren Yu(Department of Mammary Gland,Beijing Maternity Hospital of Capital Medical University,Beijing 100006,China)

机构地区：[1]首都医科大学附属北京妇产医院乳腺科,100006

出　　处：《中华实验外科杂志》2022年第4期621-624,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨5次跨膜蛋白(CD133)及POU3同源盒基因3相关长链非编码RNA(PANTR1)在乳腺癌中的表达特征及其与细胞体外增殖和侵袭能力的关系。方法将首都医科大学附属北京妇产医院乳腺科从2019年2月~2021年2月收治的50例乳腺癌患者纳入研究,分别采集乳腺癌组织以及癌旁正常组织,采用反转录聚合酶链反应(PCR)检测不同组织中CD133及PANTR1表达情况。此外,取MCF-7细胞分作对照组、CD133过表达组、PANTR1过表达组、CD133抑制组、PANTR1抑制组。分别予以Lipofectamine 2000转染,CD133过表达组予以Lipofectamine 2000以及CD133序列,PANTR1过表达组予以Lipofectamine 2000以及PANTR1序列,CD133抑制组予以Lipofectamine 2000以及si-CD133序列,PANTR1抑制组予以Lipofectamine 2000以及si-PANTR1序列。分析各组细胞增殖和侵袭能力的差异,Pum1及E2F3蛋白表达水平。组间比较采用t检验。结果乳腺癌组织CD133及PANTR1 mRNA相对表达量高于癌旁正常组织(0.021±0.001比0.006±0.001、0.016±0.002比0.005±0.001,t=75.000、74.785,P<0.01)。转染24、48、72 h时CD133过表达组细胞增殖倍数高于对照组(3.05±0.26、5.40±0.48、10.74±1.27比2.17±0.25、4.38±0.42、6.97±0.84,t=4.226、2.783、4.298,P<0.05),且转染24 h侵袭细胞数高于对照组(75.29±7.19比54.01±6.27,t=3.865,P<0.05);转染24、48、72 h时PANTR1过表达组细胞增殖倍数高于对照组(3.11±0.28、5.45±0.51、10.05±1.24比2.17±0.25、4.38±0.42、6.97±0.84,t=4.372、3.003、3.570,P<0.05),且转染24 h侵袭细胞数高于对照组(76.03±7.26比54.01±6.27,t=3.977,P<0.01)。CD133过表达组细胞中Pum1及E2F3蛋白表达量均高于对照组(4.52±0.75、3.38±0.56比1.00±0.04、1.00±0.03,t=8.118、7.351,P<0.05);PANTR1过表达组细胞中Pum1及E2F3蛋白表达量均高于对照组(4.55±0.76、3.41±0.59比1.00±0.04、1.00±0.03,t=8.079、7.066,P<0.01);而CD133抑制组细胞中Pum1及E2F3蛋白表达量均低于对照组(0.45±0.02、0.37±0.03比1.00±0.Objective To study the expression of 5-fold transmembrane protein(CD133)and POU3 homobox gene 3-related long non-coding RNA(PANTR1)in breast cancer and their relationship with cell proliferation and invasion ability in vitro.Methods A total of 50 breast cancer patients admitted to the Department of Breast Cancer,Beijing Obstetrics and Gynecology Hospital,Capital Medical University from February 2019 to February 2021 were enrolled in this study.The expression of CD133 and PANTR1 in breast cancer tissues and adjacent normal tissues was detected by reverse transcription polymerase chain reaction(PCR).In addition,McF-7 cells were divided into control group,CD133 overexpression group,PANTR1 overexpression group,CD133 inhibition group and PANTR1 inhibition group.The CD133 overexpression group was given Lipofectamine 2000 and CD133 sequence,and the PANTR1 overexpression group was given Lipofectamine 2000 and PANTR1 sequence.Lipofectamine 2000 and si-cd133 were given in CD133 inhibition group,and si-pantr1 inhibition group were given Lipofectamine 2000 and si-pantr1.The differences in proliferation and invasion ability,Pum1 and E2F3 protein expression levels in each group were analyzed.The SPSS 22.0 software was used to carry out t test.Results The relative expression levels of CD133 and PANTR1 mRNA in breast cancer tissues were higher than those in adjacent normal tissues(0.021±0.001 vs.0.006±0.001,0.016±0.002 vs.0.005±0.001,t=75.000,74.785,P<0.01).After transfection for 24,48 and 72 h,the proliferation rate of CD133 overexpression group was higher than that of control group(3.05±0.26,5.40±0.47 and 10.74±1.27 vs.2.17±0.25,4.38±0.42,6.97±0.84,t=4.226,2.783,4.298,all P<0.05),and the number of invasive cells at 24 h after transfection was greater than that of the control group(75.29±7.19 vs.54.01±6.27,t=3.865,P<0.05).After transfection for 24,48 and 72 h,the proliferation rate of PANTR1 overexpression group was higher than that of the control group(3.11±0.28,5.45±0.51 and 10.05±1.24 vs.2.17±0.25,4.38±0.42,

关 键 词：乳腺癌 长链非编码RNA 增殖 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]