长链非编码RNA ITGB1在胆囊癌组织表达及其对胆囊癌细胞增殖、迁移和侵袭的影响  

Expression of long non-coding RNA ITGB1 in gallbladder carcinoma and its effect on proliferation,migration and invasion of gallbladder carcinoma cells

作　　者：厉冰[1] 尚华[2] 梅孝臣 门中俊 翟润 孙万日[1] Li Bing;Shang Hua;Mei Xiaochen;Men ZhongJun;Zhai Run;Sun Wanri(Department of General Surgery,Nanyang Central Hospital/Department of General Surgery,Nanyang Central Hospital Affiliated to Henan University,Nanyang 473000,China;Nanyang Central Hospital,Department of Pediatric Digestive and Cardiovascular Diseases/Department of Pediatric Digestive and Cardiovascular Diseases,Nanyang Central Hospital Affiliated to Henan University,Nanyang 473000,China)

机构地区：[1]南阳市中心医院普外科/河南大学附属南阳市中心医院普外科,473000 [2]南阳市中心医院儿童消化心血管科/河南大学附属南阳市中心医院儿童消化心血管科,473000

出　　处：《中华实验外科杂志》2022年第4期653-656,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨长链非编码RNA(lncRNA)ITGB1在胆囊癌组织表达及其对胆囊癌细胞增殖、迁移和侵袭的影响。方法选取2018年8月到2020年8月我院收集的98例胆囊癌及其癌旁组织作为研究对象,采用荧光定量聚合酶链反应(PCR)分析胆囊癌组织和癌旁组织lncRNA ITGB1表达水平;胆囊癌细胞系GBC-SD随机分为lncRNA对照组和lncRNA ITGB1 KD组,采用细胞计数试剂盒(CCK-8)测定两组细胞增殖,采用划痕实验和Transwell实验分析两组细胞的迁移和侵袭能力;采用蛋白质印迹法(Western blot)分析两组细胞增殖细胞核抗原(PCNA)、黏着斑激酶(FAK)、MMP-13蛋白表达水平。组间比较采用t检验。结果胆囊癌组织lncRNA ITGB1表达水平(2.72±0.37)明显高于癌旁组织lncRNA ITGB1表达水平(1.06±0.18),差异有统计学意义(t=38.931,P<0.05)。lncRNA对照组细胞24 h和48 h吸光度值(1.37±0.04、2.16±0.04)明显高于lncRNA ITGB1 KD组细胞吸光度值(1.05±0.04、1.53±0.03),差异有统计学意义(t=12.931、27.200,P<0.05)。lncRNA对照组细胞划痕愈合率[(84.21±6.30)%]明显高于lncRNA ITGB1 KD组细胞划痕愈合率[(41.80±4.92)%],差异有统计学意义(t=11.860,P<0.05)。,lncRNA对照组细胞迁移数量[(148.60±13.94)个]明显高于lncRNA ITGB1 KD组细胞迁移数量[(100.20±13.12)个],差异有统计学意义(t=5.653,P<0.05)。lncRNA对照组细胞PCNA、FAK和MMP-13蛋白表达水平(0.98±0.09、1.21±0.12、1.09±0.11)明显高于lncRNA ITGB1 KD组细胞(0.44±0.05、0.79±0.08、0.45±0.09),差异有统计学意义(t=10.531、5.841、9.101,P<0.05)。结论lncRNA ITGB1在胆囊癌组织中呈高表达,通过调节PCNA、FAK和MMP-13蛋白的表达水平,促进了肿瘤细胞增殖、迁移和侵袭等过程。Objective To investigate the expression of long non-coding RNA(lncRNA)ITGB1 in gallbladder carcinoma and its effect on the proliferation,migration and invasion of gallbladder carcinoma cells.Methods A total of 98 cases of gallbladder cancer tissues and the adjacent tissues collected in our hospital from August 2018 to August 2020 were selected as the research objects.The expression levels of lncRNA ITGB1 in gallbladder cancer and adjacent tissues were analyzed by fluorescence quantitative polymerase chain reaction(PCR).Gallbladder cancer cell line GBC-SD was randomly divided into lncRNA control group and lncRNA ITGB1 group.The cell proliferation of the two groups was measured by cell counting kit-8(CCK-8)assay.The migration and invasion ability of the two groups was analyzed by scratch test and Transwell test.The expression levels of proliferating cell nuclear antigen(PCNA),focal adhesion kinase(FAK)and matrix metalloproteinase-13(MMP-13)were detected by Western blotting.The data with normal distribution were expressed as mean±standard deviation.Results The expression level of lncRNA ITGB1 in gallbladder cancer tissues(2.72±0.37)was significantly higher than that in adjacent tissues(1.06±0.18,t=38.931,P<0.05).The absorbance value of cells in lncRNA control group at 24 h and 48 h(1.37±0.04,2.16±0.04)was significantly higher than that in lncRNA ITGB1 KD group(1.05±0.04,1.53±0.03,t=12.931,7.200,P<0.05).he cell scratch healing rate of lncRNA control group[(84.21±6.30)%]was significantly higher than that of lncRNA ITGB1 KD group[(41.80±4.92)%,t=11.860,P<0.05].The number of migrating cells in lncRNA control group[(148.60±13.94)]was significantly greater than that in lncRNA ITGB1 KD group[(100.20±13.12),t=5.653,P<0.05].The expression levels of PCNA,FAK and MMP-13 proteins in lncRNA control group(0.98±0.09,1.21±0.12,1.09±0.11)were significantly higher than those in lncRNA ITGB1 KD group(0.44±0.05,0.79±0.08,0.45±0.09,t=10.531,5.841,9.101,P<0.05).Conclusion LncRNA ITGB1 is highly expressed in gallbladder c

关 键 词：长链非编码RNA 胆囊癌 增殖 迁移 侵袭 

分 类 号：R735.8[医药卫生—肿瘤]