7个指标的微波酒制白屈菜炮制工艺研究  

Study on Processing Technology of Seven Indexes of Microwave Wine for Chelidonium Majus

作　　者：黄爽 李瑞海[1] 贾天柱[1] Huang Shuang;Li Ruihai;Jia Tianzhu(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《亚太传统医药》2022年第5期61-65,共5页Asia-Pacific Traditional Medicine

基　　金：国家药典委员会项目(20180604005)。

摘　　要：目的:以别隐品碱等7个生物碱为指标的正交法优选酒制白屈菜最佳炮制工艺。方法:采用HPLC法,以微波酒制白屈菜中原阿片碱、别隐品碱等7个生物碱总碱含量为考察指标,以黄酒用量、焖润时间、火力大小和炮制时间为考察因素,各取3个水平,采用正交试验,优选微波酒制法的最佳工艺。结果:所建立的HPLC法对7种对照品分离度较好,方法学考察结果符合相关规定。微波酒制白屈菜最佳炮制工艺为黄酒用量40%,焖润1h,60%火力下炮制3min。结论:优选的微波酒制白屈菜炮制工艺合理可行,可为白屈菜饮片的生产提供试验依据。该炮制工艺尤其对白屈菜中别隐品碱、原阿片碱、白屈菜碱、盐酸黄连碱4个成分的提取效果显著,可明显增加别隐品碱等4个成分的含量,提示本酒制工艺具有一定的增效作用,可为白屈菜减毒增效研究奠定基础。Objective:To optimize the best processing technology of the liquor to Chelidonium Majus by orthogonal method,which was based on 7alkaloids such as the alkaloids of the cryptophantine.Methods:The total alkaloids content of 7alkaloids,such as opilline and cryptophantine,were used as the indexes by HPLC.The three levels were taken as the factors,such as the dosage of yellow wine,the time of drenched,the size of fire and the processing time.The optimum technology of microwave wine was optimized by orthogonal test.Results:The HPLC method was good for the separation of 7control products,and the results of the methodology were in line with the relevant regulations.The best processing technology of microwave wine was 40%of rice wine,1hour of braising and 3min under 60%fire.Conclusion:The optimum microwave wine processing technology was reasonable and feasible,which can provide experimental basis for the production of the pieces of Chelidonium Majus.In particular,the factors of extraction of four components,such as cryptophantine,etc,were significant.The content of four components such as cryptophantine,etc,could be increased obviously under the processing conditions,which suggested that the wine making process had a certain synergistic effect,which laid a foundation for the study of reducing the efficiency of Dioscorea.

关 键 词：白屈菜 微波 酒制 生物碱 正交法 

分 类 号：R283.3[医药卫生—中药学]