基于生物信息学分析筛选结肠癌关键基因  

Screening Key Genes of Colon Cancer Based on Bioinformatics Analysis

作　　者：崔忠泽 何双[1] 路丽祯 吴淑华[1] CUI Zhong-ze;HE Shuang;LU Li-zhen;WU Shu-hua(Department of Pathology,Affiliated Hospital,Binzhou Medical University,Binzhou 256600,Shandong,China)

机构地区：[1]滨州医学院附属医院病理科,山东滨州256600

出　　处：《医学信息》2022年第10期1-7,共7页Journal of Medical Information

摘　　要：目的鉴定可能参与结肠癌发生发展的预后相关枢纽基因和免疫相关基因。方法从TCGA数据库和GEO数据库(GSE41657、GSE74602、GSE106582和GSE110224)中下载结肠癌数据集,鉴定共同差异表达基因,并对其进行功能富集分析、预后相关基因筛选、免疫相关基因筛选、蛋白相互作用网络构建,进一步分析预后相关枢纽基因和免疫相关基因的表达量、免疫组化和功能富集。结果共筛选102个共同差异基因,其中预后相关基因15个,免疫相关基因6个,枢纽基因16个,选取预后相关枢纽基因(SLC17A4)和免疫相关基因(NR3C2、ETFDH、SCG2)作为目标基因进一步分析,发现ETFDH主要表达于细胞质,NR3C2在细胞核和细胞质中大量表达,SCG2在细胞质中有少量表达,SLC17A4主要表达于细胞膜,上述基因在癌组织中均低表达,富集分析显示其与诸多结肠癌相关功能关联,其中免疫相关基因与结肠癌免疫密切相关并总体呈正相关。结论15个预后相关基因有望作为生物标记物来预测结肠癌预后。SLC17A4在结肠癌中作为预后相关枢纽基因可能发挥重要功能,NR3C2、ETFDH、SCG2作为免疫相关预后基因可能在调控结肠癌肿瘤免疫过程中发挥着重要作用,这为探索结肠癌的分子机理提供了新的思路。Objective To identify the prognostic hub genes and immune related genes that may be involved in the development of colon cancer.Methods The data sets of colon cancer were downloaded from TCGA database and GEO database(GSE41657,GSE74602,GSE106582 and GSE110224),and the common differentially expressed genes were identified.Functional enrichment analysis,prognosis-related gene screening,immune-related gene screening and protein-protein interaction network construction were performed on the common differentially expressed genes.The expression levels,immunohistochemistry and functional enrichment of prognosis-related hub genes and immune-related genes were further analyzed.Results A total of 102 common differentially expressed genes were screened,including 15 prognostic-related genes,6 immune-related genes and 16 hub genes.The prognostic-related hub genes(SLC17A4)and immune-related genes(NR3C2,ETFDH,SCG2)were selected as target genes for further analysis.It was found that ETFDH was mainly expressed in the cytoplasm,NR3C2 was highly expressed in the nucleus and cytoplasm,SCG2 was slightly expressed in the cytoplasm,SLC17A4 was mainly expressed in the cell membrane,and the above genes were lowly expressed in cancer tissues.Enrichment analysis showed that they were associated with many colon cancer-related functions.Among them,immune-related genes were closely related to colon cancer immunity and are generally positively correlated.Conclusion Fifteen prognostic genes are expected to be used as biomarkers to predict the prognosis of colon cancer.SLC17A4 may play an important role as a prognostic pivotal gene in colon cancer,while NR3C2,ETFDH and SCG2 as immunorelated prognostic genes may play an important role in the regulation of colon cancer tumor immunity,which provides a new idea for exploring the molecular mechanism of colon cancer.

关 键 词：结肠癌 免疫相关基因 预后相关基因 功能富集 

分 类 号：R735.3[医药卫生—肿瘤]