转录因子核因子κB和IKK在慢加急性肝衰竭中的临床诊断价值  

Clinical Diagnostic Value of NF-κB and IKK Protein in STING-IRF3 Pathway in Chronic and Acute Liver Failure

作　　者：袁慧姣[1] 韩波[1] YUAN Hui-Jiao;HAN Bo(Department of Clinical Laboratory,the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学附属医院检验科,沈阳110032

出　　处：《中国药物经济学》2022年第3期31-34,共4页China Journal of Pharmaceutical Economics

基　　金：辽宁省教育厅科学技术研究项目(L201917)。

摘　　要：目的探讨在STING-IRF3通路中转录因子核因子κB和IKK在慢加急性肝衰竭(ACLF)中的表达及其与患者临床特征和预后的相关性。方法将2014年3月至2018年11月于辽宁中医药大学附属医院就诊的144例ACLF患者作为研究对象,收集患者的血液、尿液与术后切除的肝部样本作为试验组,同时选取144例正常人的血液、尿液与肝部组织作为对照组,采用蛋白质免疫印迹法检测不同样本中核因子κB和IKK蛋白表达量与磷酸化水平变化情况,以及其与患者临床特征和预后的相关性。结果核因子κB蛋白在患者的肝部、血液与尿液中的表达量不变(P>0.05),而磷酸化水平明显升高(P<0.05);IKK蛋白在患者的肝部、血液以及尿液中的蛋白表达量与磷酸化蛋白表达量显著升高(P<0.05);核因子κB和IKK蛋白表达均为阳性的患者,其4年的生存率为5.89%;核因子κB蛋白表达为阳性,IKK蛋白表达为阴性的患者,其4年的生存率为20.12%;核因子κB蛋白表达为阴性,IKK蛋白表达为阳性的患者,其4年的生存率为25.18%。结论STING-IRF3通路中转录因子核因子κB和IKK对ACLF具有正向调控作用。Objective To investigate the expression and clinical significance of transcription factor NF-κB(nuclear factor kappa-B)and IKK(kappa-B kinase)in acute chronic liver failure(ACLF)within the STING-IRF3 pathway correlation between features and prognosis.Methods A total of 144 patients with chronic acute liver failure admitted to the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from March 2014 to November 2018 were selected as the research objects.The blood,urine and postoperative liver samples of the patients were collected as experimental group.The blood,urine and liver tissues of 144 normal subjects were selected as control group.Western blotting was used to detect the total protein content and phosphorylation level changes of NF-κB and IKK in different samples,as well as their clinical characteristics and prognosis.Results The total protein content level of NF-κB protein in the liver,blood and urine of patients were normal(P>0.05)but its’phosphorylation protein was significantly increase(P<0.05);the total protein of IKK protein and phosphorylation IKK in the liver,blood and urine of patients was significantly increased(P<0.05);patients with positive expression of NF-κB and IKK protein with a 4-year survival and the survival rate was 5.89%;patients with positive NF-κB protein expression and negative IKK protein expression with a 4-year survival rate of 20.12%;patients with negative NF-κB protein expression and positive IKK protein expression with a 4-year survival rate and the survival rate was 25.18%.Conclusion The transcription factors NF-κB and IKK in the STING-IRF3 pathway have a positive regulatory effect on chronic-onset acute liver failure.

关 键 词：STING-IRF3信号通路 核因子κB蛋白 IKK蛋白 慢加急性肝衰竭 

分 类 号：R575.3[医药卫生—消化系统]