姜黄素和/或有氧运动对血脂紊乱大鼠肠道机能的调节作用  

Effects of curcumin and/or aerobic exercise on intestinal function in dyslipidemia rats

作　　者：王丽[1] 吉喆 董丽 路昕 秦菲 胡戈[4] 周海涛 曹建民[1] WANG Li;JI Zhe;DONG Li;LU Xin;QIN Fei;HU Ge;ZHU Haitao;CAO Jianmin(Beijing Sport University,Beijing 100084,China;Beijing Union University,Beijing 100101;Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191;Changzhou University,Changzhou 213164)

机构地区：[1]北京体育大学,北京100084 [2]北京联合大学,北京100101 [3]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100191 [4]常州大学,江苏常州213164

出　　处：《中国实验动物学报》2022年第2期208-215,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京体育大学2020年中央高校基本科研业务费专项资金资助(2020033);北京联合大学科研项目(ZK30202104)。

摘　　要：目的探讨姜黄素(curcumin)和/或有氧运动对血脂紊乱大鼠肠道机能的调节作用。方法5周龄雄性SPF级SD大鼠40只,随机分为普通饮食+安静组(CON组)、高脂饮食+安静组(HDC组)、高脂饮食+姜黄素+安静组(HDM组)、高脂饮食+有氧运动组(HDE组)和高脂饮食+姜黄素+有氧运动组(HDME组)。高脂饮食各组以高脂饲料喂养,普通饮食组以维持饲料喂养。第3周起,HDM、HDME组以200 g/(kg·d)姜黄素灌胃,其余组以等体积0.5%羧甲基纤维素钠灌胃;HDE、HDME组进行运动强度为70%~75%最大摄氧量的有氧运动,其余组无任何运动干预。6周干预结束后24 h,腹主动脉取血,取盲肠组织、盲肠内容物。全自动生化分析仪检测血脂水平;HE染色后观察大鼠盲肠组织形态;Western Blot法检测盲肠组织闭锁小带蛋白-1(zona occludens-1,ZO-1)蛋白质表达水平;16S rDNA基因测序技术检测盲肠内容物肠道菌群分布。结果姜黄素和/或有氧运动均可降低血脂紊乱大鼠血清总胆固醇、甘油三酯(P<0.05);上调盲肠组织ZO-1蛋白质表达水平(P<0.05)并改善组织形态;改善肠道菌群的多样性和均匀度(P<0.05)。结论姜黄素和/或有氧运动均可通过有效调节肠道菌群,改善血脂紊乱大鼠肠黏膜屏障完整性及肠道机能。其中联合干预效果最佳。Objective To investigate the effects of curcumin and/or aerobic exercise on intestinal function in dyslipidemic rats.Methods Forty specific-pathogen free male Sprague Dawley rats were divided randomly into the following groups:normal diet+quiet(CON group),high-fat diet+quiet(HDC group),high-fat diet+curcumin+quiet(HDM group),high-fat diet+aerobic exercise(HDE group),and high-fat diet+curcumin+aerobic exercise(HDME group).All the high-fat diet groups received a high-fat diet and the CON group received a maintenance diet.From the third week,the HDM and HDME groups were also given 200 g/(kg·d)curcumin,and the other groups were given 0.5%sodium carboxymethyl cellulose.The HDE and HDME groups underwent aerobic exercise intervention with 70%~75%maximal oxygen uptake,while the other groups did not undergo any exercise intervention.Twenty-four hours after the 6 weeks intervention,blood was taken from the abdominal aorta,and cecal tissue and contents were collected.Blood lipid levels were detected using an automatic biochemical analyzer.Cecal morphology was observed by hematoxylin and eosin staining.Expression of zonula occludens-1(ZO-1)protein in cecal tissue was detected by western blot.The distribution of the intestinal flora in the cecal contents was detected by 16 S rDNA sequencing.Results Curcumin and/or aerobic exercise significantly decreased serum total cholesterol and triglyceride levels(P<0.05),up-regulated ZO-1 expression in cecal tissue(P<0.05),attenuated the cecal histopathological changes,and improved the diversity and evenness of the intestinal flora in dyslipidemic rats(P<0.05).Conclusions Curcumin and/or aerobic exercise can reduce blood lipid levels in dyslipidemic rats,regulate the cecal intestinal flora,protect the integrity of the intestinal mucosal barrier,and adjust intestinal function,with the combination of curcumin and aerobic exercise having the greatest effect.

关 键 词：姜黄素 有氧运动 血脂紊乱 肠道菌群 闭锁小带蛋白-1 

分 类 号：Q95-33[生物学—动物学]