视网膜类器官衍生物的研究应用  

An overview on applications of retinal organoid derivatives

作　　者：陈浩浩(综述) 金子兵(审校) CHEN Haohao;JIN Zibing(Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Ophthalmology and Visual Science Key Lab,Beijing 100005,China)

机构地区：[1]北京市眼科研究所,首都医科大学附属北京同仁医院,北京同仁眼科中心,北京市眼科学与视觉科学重点实验室,北京100005

出　　处：《眼科学报》2022年第4期255-270,共16页Eye Science

摘　　要：视网膜退行性疾病的种类繁多、患病人口基数大,该病特征为终末期严重的视网膜细胞丢失。视网膜类器官(retinal organoid,RO)可通过3D干细胞体外分化培养技术大量获取,并拥有完整的各亚型视网膜细胞和经典的视网膜分层结构。因此,RO可作为最佳的视网膜退行性疾病建模方法之一,以便于发现潜在致病机制。目前,RO衍生物已被广泛用于视网膜细胞替代治疗的动物实验和临床研究,具体的成效参差不齐,可能的影响因素包括移植细胞数量、移植时间窗、移植工具等。随着RO相关研究的快速发展,视网膜退行性疾病在分子和个体上的诊断和治疗将进一步完善。Retinal degenerative diseases,characterized by severe retinal cell loss at the end stage,are of various kinds and haunt vast amounts of patients. Retinal organoid(RO) with complete retinal cell subtypes and classic retinal stratification structures can be obtained in large quantities through stem cells in vitro 3 D differentiation and culture method.Therefore,RO can serve as one of the best ways for retinal degenerative disease modeling to facilitate the decipherment of underlying pathogenic mechanisms.At present,RO derivatives have been widely used in animal experiments and clinical studies of retinal cell replacement therapy with varying results possibly affected by cell quantity,time window,or tools in terms of transplantation.With the booming progress of ROrelated research,the diagnosis and treatment on molecular and individual level for retinal degenerative diseases will be further improved.

关 键 词：视网膜 干细胞 类器官 基因编辑 细胞替代治疗 

分 类 号：R73[医药卫生—肿瘤]