宫颈病变中NOB1、miR-21的表达及与高危型HPV感染关系  

The expression of NOB1 and miR-21 in cervical lesions tissues of women and its association with the cervical high-risk human papillomavirus infection of the women

作　　者：李宏[1] 祁美霞[1] 纪桢 吴小蕊 LI Hong;QI Meixia;JI Zhen;WU Xiaorui(The Fifth Central Hospital of Tianjin,Tianjin,300450)

机构地区：[1]天津市第五中心医院,300450

出　　处：《中国计划生育学杂志》2022年第2期457-461,共5页Chinese Journal of Family Planning

摘　　要：目的:探讨宫颈病变中NOB1、miR-21的表达及与高危型人乳头瘤病毒(HPV)感染的相关性。方法:选取2018年1月-2020年5月本院就诊的宫颈病变患者138例临床资料进行回顾性分析,其中宫颈癌52例(宫颈癌组)、宫颈鳞状上皮内病变54例(上皮内病变组)、慢性宫颈炎32例(宫颈炎组),对比各组NOB1阳性率、阳性细胞比例和染色强度综合评分、miR-21表达量及HR-HPV阳性率及DNA负荷量;分析NOB1、miR-21与高危型HPV感染的相关性;评估NOB1、miR-21诊断宫颈癌和预测高危型HPV感染效能。结果:NOB1阳性率、阳性细胞的比例和染色强度综合评分及miR-21表达量宫颈癌组、上皮内病变组、宫颈炎组依次降低;宫颈癌组HR-HPV阳性率、HR-HPV DNA负荷量中100~1000、≥1000占比高于另外两组(P<0.05),1~100占比3组无差异(P>0.05)。Kendall’s tau-b相关性分析显示,NOB1阳性细胞比例和染色强度综合评分、miR21表达量与HR-HPV DNA负荷量呈正相关(r=0.137、0.143,均P<0.05)。多元逐步回归分析显示,NOB1阳性细胞比例和染色强度综合评分为HR-HPV阳性率的影响因素。ROC曲线分析显示,NOB1阳性细胞比例和染色强度综合评分、miR-21诊断宫颈癌的AUC分别为0.950、0.975(P<0.05),预测高危型HPV感染的AUC分别为0.739、0.712(P<0.05)。结论:宫颈癌患者NOB1阳性、miR-21呈高表达,且表达与HR-HPV DNA负荷量呈正相关,对宫颈癌诊断和预测高危型HPV感染有一定价值。Objective: To explore the expressions of NOB1 and miR-21 in cervical lesions tissues of women, and to study its association with the cervical high-risk human papillomavirus(HR-HPV) infection of the women. Methods: The clinical data of 138 women with cervical lesions from January 2018 to May 2020 were selected for retrospective analysis. These women included 52 cases with cervical cancer in group A, 54 cases with cervical squamous intraepithelial lesions in group B, and 32 cases with chronic cervicitis group C. The positive rate of NOB1, the proportion of positive cells, and the comprehensive score of staining intensity of the cells, the expression level of miR-21, the positive rate of HR-HPV, and the DNA load of the women were compared among the three groups. The correlation between the positive rate of NOB1 and the miR-21 level of the women with their HR-HPV infection was analyzed. The efficiency of the positive rate of NOB1 and the miR-21 level of the women for diagnosing the cervical cancer and for predicting their HR-HPV infection were evaluated. Results: The positive rate of NOB1, the proportion of positive cells, the comprehensive score of staining intensity of the cells, and the expression level of Mir-21 of the women in group A, group B, and group C had decreased gradually. The HR-HPV positive rate, and the proportions of HR-HPV DNA load of 100-1000 and ≥1000 of the women in group A were significantly higher than those of the women in the other two groups(P<0.05), but there was no significant difference in the proportion of HR-HPV DNA load of 1-100 of the women among the three groups(P>0.05). Kendall’s TaU-B correlation analysis showed that the proportion of NOB1 positive cells,the comprehensive score of staining intensity of the cells,and the miR21 expression level of the women with HR-HPV infection were positively correlated with their HR-HPV DNA load(r=0.137,0.143,all P<0.05).Multiple stepwise regression analysis showed that the proportion of NOB1 positive cells and the comprehensive score of stain

关 键 词：宫颈病变 宫颈癌 NOB1 MIR-21 高危型人乳头瘤病毒 相关性 预测 

分 类 号：R737.33[医药卫生—肿瘤]