脐带来源的间充质干细胞对人卵巢皮质异种移植模型血管生成的影响  

Influence of umbilical cord-derived mesenchymal stem cells on the angiogenesis of the model of human ovary cortex after xenotransplantation

作　　者：曹晓敏[1] 刘丽[1] 邸建永[1] 方祺[1] 徐凤琴[1] CAO Xiaomin;LIU Li;DI Jinyong;FANG Qi;XU fengqin(The First Central Hospital of Tianjin,Tianjin,300192)

机构地区：[1]天津市第一中心医院,300192

出　　处：《中国计划生育学杂志》2022年第5期976-979,共4页Chinese Journal of Family Planning

摘　　要：目的:探讨脐带来源的间充质干细胞(UC-MSCs)对人卵巢皮质异种移植模型血管生成的影响。方法:对1例捐赠的卵巢皮质进行玻璃化冷冻保存。复苏后移植到重症联合免疫缺陷(SCID)小鼠背部皮下,配对分为UC-MSCs组和对照组各10只。移植后3,7,14d观察移植物局部血管重建情况,比较各组的卵泡存活、凋亡、微血管密度和微循环血流量情况。结果:移植后7d和14d UC-MSCs组卵泡形态正常率较对照组明显升高(50.2%比38.5%,45.4%比34.0%),卵巢组织中凋亡卵泡占比低于对照组(19.0%比27.2%,31.5%比46.3%)(均P<0.05)。移植后3,7,14d UC-MSCs组的微血管密度均高于对照组(P<0.05)。移植后7,14d UC-MSCs组移植物局部的血流灌流量均高于对照组[(106.4±12.2)PU比(87.8±11.9)PU,(124.5±18.4)PU比(99.3±16.6)PU](P<0.05)。结论:UC-MSCs可加速卵巢移植后局部微血管的生成,降低移植后卵泡发生凋亡比率。Objective: To investigate the influence of umbilical cord derived mesenchymal stem cells(UC-MSCs) on the angiogenesis of the model of human ovary cortex after xenotransplantation. Methods: One sample of donated ovarian cortex was cryopreserved by vitrification. After resuscitation, this ovarian cortex was subcutaneously transplanted into the back of 20 mice with severe case and immunodeficiency(SCID). These mice were divided into two groups(10 cases in each group). The mice in the study group were given UC-MSCs treatment, and the mice in the control group were not given UC-MSCs treatment. The local vascular reconstruction of the transplanting local of the mice were observed in the 3 rd day, the 7 th day, and the 14 th day after transplantation of human ovary cortex. The follicle survival and apoptosis, and the situations of the microvascular density and microcirculation blood flow of the mice were compared between the two groups. Results: The normal follicular morphology rate of the mice in the study group in the 7 th day or in the 14 th day after transplantation(50.2% or 45.4%) was significantly higher than that(38.5% or 34.0%) of the mice in the control group(P<0.05). The proportion of apoptotic follicles in the ovarian tissue of the mice in the study group in the 7 th day or in the 14 th day after transplantation(19.0% or 31.5%) was significantly lower than that(27.2% or 46.3%) of the mice in the control group(P<0.05). The microvascular density of the mice in the study group in the 3 rd day, the 7 th day, or the 14 th day after transplantation was significantly higher than that of the mice in the control group(P<0.05). The value of local perfusion of blood flow of the transplanting locals of the mice in the study group in the 7 th day or in the 14 th day after transplantation(106.4± 12.2 PU or 124.5± 18.4 PU) was significantly higher than that(87.8± 11.9 PU or 99.3± 16.6 PU) of the mice in the control group(P<0.05). Conclusion: UC-MSCs can accelerate the formation of microvessels of the transplanting local

关 键 词：卵巢移植 微循环重建 间充质干细胞 脐带 血管生成 

分 类 号：R-332[医药卫生]