分子标记筛选抗马铃薯Y病毒种质资源研究  

Studies on Screening Potato Germplasm Resources with Resistance to Potato Virus Y by Molecular Marker

作　　者：蒋伟 卢丽丽[1] 包丽仙[1] 李秀芬 杨绍英 杨荣凯 杨美青 李先平[1] JIANG Wei;LU Lili;BAO Lixian;LI Xiufen;YANG Shaoying;YANG Rongkai;YANG Meiqing;Li Xianping(Industrial Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan,China;Baga Township Agricultural Integrated Service Center,Yanshan County,Wenshan Prefecture,Yunnan Province,Wenshan 663109,Yunnan,China;Agricultural and Rural Comprehensive Service Center,Huanian Town,Esan County,Yuxi City,Yunnan Province,Yuxi 653203,Yunnan,China;Agricultural Technology Extension Center of Yingjiang County Agriculture and Rural Affairs Bureau,Dehong Prefecture,Yunnan Province,Dehong 679300,Yunnan,China;College of Pharmacy,Baotou Medical College,Baotou 014040,Inner Mongolia,China)

机构地区：[1]云南省农业科学院经济作物研究所,云南昆明650205 [2]云南省文山州砚山县八嘎乡农业综合服务中心,云南文山663109 [3]云南省玉溪市峨山县化念镇农业农村综合服务中心,云南玉溪653203 [4]云南省德宏州盈江县农业农村局农业技术推广中心,云南德宏679300 [5]包头医学院药学院,内蒙古包头014040

出　　处：《中国蔬菜》2022年第5期16-22,共7页China Vegetables

基　　金：国家重点研发计划项目(2017YFE0104700)。

摘　　要：为快速有效筛选抗马铃薯Y病毒种质,加快抗病毒新品种的选育进程,本试验对引自美国的9个薯条加工型马铃薯杂交组合后代进行PVY抗性基因分子标记筛选,并通过PVY人工接种鉴定评价分子标记预测的准确性。研究结果表明:检测的9个杂交组合852个株系中,共有4个组合163个株系中含有抗性基因Ry_(adg)分子标记RYSC3,占比19.13%;7个组合330个株系中含有抗性基因Ry_(sto)分子标记YES3-3A,占比38.73%。其中,2个组合42个株系中同时检测到2种分子标记。人工接种PVY抗性鉴定结果表明,含有分子标记的株系接种后表现为抗PVY的比例为92.73%;不含有分子标记的株系接种后表现为感PVY的比例为28.57%。PVY抗性基因分子标记RYSC3和YES3-3A能够较准确地鉴定马铃薯PVY抗性资源,有助于早期选育抗马铃薯Y病毒种质资源。同时,本试验筛选获得10份高产、稳产的抗PVY薯条加工型马铃薯资源,可以为薯条加工型马铃薯新品种的选育提供具有PVY抗性和适于薯条加工的亲本材料。In order to rapidly and effectively screen germplasm resources resistant to potato virus Y,and speed up the breeding process of new potato varieties resistant to virus,this experiment screened progenies from 9 potato hybridized combinations of processing type introduced from USA using PVY resistant gene molecular marker;and identified/evaluated the prediction accuracy of molecular marker by PVY artificial inoculation.The results showed that among 852 lines in 9 hybridized combinations,163 lines from 4 combinations contained RYSC3 molecular marker with resistant gene Ry_(adg),accounting for 19.13%;330 lines in 7 combinations contained YES3-3A molecular marker with resistanct gene Ry_(sto),accounting for 38.73%.Among them,2 kinds molecular marker were simultaneously detected in 42 lines from 2 combinations.The results of PVY resistance identification by artificial inoculation showed that 92.73% of the lines containing molecular marker were resistant to PVY after inoculation.28.57% of the lines without molecular marker showed sensitivity to PVY after inoculation.RYSC3 and YES3-3A molecular markers with PVY resistance genes could more accurately identify potato resources resistant to PVY.These 2 molecular markers were helpful for early selection and breeding of potato germplasm resources resistant to PVY.At the same time,this experiment obtained 10 potato germplasm resources of processing type with high and stable production and resistant to PVY.These results had provided parent materials for breeding new potato varieties of processing type and yet resistant to PVY.

关 键 词：马铃薯 分子标记 马铃薯Y病毒 抗性基因 资源筛选 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]