NF-κB Apaf-1蛋白在胃癌组织中的表达及意义  

Expression and Significance of NF-κB Protein and Apaf-1 Protein in Gastric Cancer

作　　者：杨波[1] 杨涛[1] 杨东霞 张歆萍[1] 孙海宁 李佳欣 YANG Bo;YANG Tao;YANG Dongxia(Chengde Central Hospital, Hebei Chengde 067000,China)

机构地区：[1]河北省承德市中心医院,河北承德067000 [2]承德医学院病理教研室,河北承德067000

出　　处：《河北医学》2022年第5期766-770,共5页Hebei Medicine

基　　金：河北省研究生创新资助项目,(编号:CXZZSS2020123)。

摘　　要：目的:探讨核转录因子-κB(Nuclear factor kappa-B,NF-κB)和凋亡蛋白酶活化因1(Apoptotic protease activating factor-1,Apaf-1)在胃癌组织中的表达及意义,为胃癌的临床治疗开辟新思路。方法:收集50例胃癌患者的胃癌组织及其癌旁正常组织,采用免疫组化SP法检测NF-κB和Apaf-1蛋白的表达情况,分析二者与临床病理特征的关系和二者间相关性。结果:NF-κB蛋白在胃癌组织中的表达高于癌旁正常组织,而Apaf-1蛋白的表达低于癌旁组织,二者蛋白表达变化与分化程度、TNM分期、浸润深度及淋巴转移均有关(P<0.05)。相关性分析显示胃癌组织中NF-κB蛋白和Apaf-1蛋白表达为负相关(rs=-0.36,P<0.05)。结论:胃癌组织中NF-κB蛋白和Apaf-1蛋白表达异常,参与了胃癌的发生、发展,有望为胃癌的诊断及治疗提供参考。Objective:To explore the expression and significance of nuclear factor-κB(NF-κB)and apoptotic protease activating factor-1(Apaf-1)in gastric cancer tissues,and to open up new ideas for the clinical treatment of gastric cancer.Methods:A total of 50 experimental specimens were collected,including gastric cancer tissues and corresponding paracancerous tissues in the same period.The expressions of NF-κB and Apaf-1 proteins were detected by immunohistochemistry SP method,and the relationship between the two and clinical pathological characteristics as well as their correlation were analyzed.Results:The expression level of NF-κB protein in gastric cancer tissues was higher than that of adjacent tissues,while the expression level of Apaf-1 protein was lower than that of adjacent tissues.The protein expression changes of the two proteins were related to the differentiation degree,TNM stage,infiltration depth and lymphatic metastasis(P<0.05).Correlation analysis showed that the expressions of NF-κB protein and Apaf-1 protein in gastric cancer tissues were negatively correlated(rs=-0.36,P<0.05).Conclusion:The abnormal expression of NF-κB and Apaf-1 proteins in gastric cancer tissues may be involved in the occurrence and development of gastric cancer.

关 键 词：胃癌 核转录因子-ΚB 凋亡蛋白酶活化因子1 免疫组化SP法 

分 类 号：R73[医药卫生—肿瘤]