Reversal of multidrug resistance and antitumor promoting activity of 3-oxo-6β-hydroxy-β-amyrin isolated from Pistacia integerrima  

作　　者：ABDUR RAUF SAUD BAWAZEER MUSLIM RAZA EMAN EL-SHARKAWY MD.HABIBUR RAHMAN MOHAMED A.EL-ESAWI GHIAS UDDIN BINA SSIDDIQUI ANEES AHMED KHALIL JOSEPH MOLNAR AKOS CSONKA DIÁNA SZABÓ HAROON KHAN MOHAMMAD SMUBARAK TAIBI BEN HADDA MUDYAWATI KAMARUDDIN SEEMA PATEL 

机构地区：[1]Department of Chemistry,University of Swabi,Anbar,23561,Khyber Pakhtunkhwa Pakistan [2]Department of Pharmaceutical Chemistry,Faculty of Pharmacy,Umm Al-Qura University,Makkah,24211,Saudi Arabia [3]State Key Laboratory of Chemical Resource Engineering,Beijing University of Chemical Technology,Beijing,100029,China [4]Chemical Departments,Faculty of Science,Northern Borders University,Arar,91431,Saudi Arabia [5]Department of Pharmacy,Southeast University,Banani-1213,Dhaka,Bangladesh [6]Department of Global Medical Science,Wonju College of Medicine,Yonsei University,Seoul,South Korea [7]Botany Department,Faculty of Science,Tanta University,Tanta,31527,Egypt [8]Institute of Chemical Sciences,University of Peshawar,Peshawar,25120,Pakistan [9]H.E.J.Research Institute of Chemistry,International Center for Chemical and Biological Sciences,University of Karachi,Karachi,75270,Pakistan [10]University Institute of Diet and Nutritional Sciences,Faculty of Allied Health Sciences,The University of Lahore,Lahore,54000,Pakistan [11]Department of Medical Microbiology and Immunobiology,Faculty of Medicine,University of Szeged,Szeged,6700,Hungary [12]Department of Oto-Rhino-Laryngology and Head-Neck Surgery,Faculty of Medicine,University of Szeged,Szeged,6700,Hungary [13]Department of Pharmacy,Abdul Wali Khan University,Mardan,23200,Pakistan [14]Department of Chemistry,The University of Jordan,Amman,11942,Jordan [15]LCM Laboratory,Faculty of Sciences,Mohamed First University,Oujda,60000,Morocco [16]Institution of Health Science of Tahirah Al Baeti Bulukumba,South Sulawesi,92352,Indonesia [17]Bioinformatics and Medical Informatics Research Center,San Diego State University,San Diego,92182,USA

出　　处：《BIOCELL》2021年第1期139-147,共9页生物细胞（英文）

基　　金：funded by Higher Education commission,Pakistan(HEC)(Grant No.NRPU649).

摘　　要：The bioactive triterpenoid 3-oxo-6-β-hydroxy-β-amyrin(1)has been isolated from multiple plant sources.In this study,chloroform fraction of Pistacia integerrima extract was processed for the isolation of the compound.The compound identity was confirmed by advanced spectroscopy technique.X-ray crystallography was applied for molecular structure confirmation.In addition,compound 1 was screen for its activity on reversal of MDR(multidrug resistance)mediated by P-gp(P-glycoprotein).This was accomplished by using rhodamine123 exclusion on multidrug-resistant human ABCB1 gene transfected mouse T-lymphoma cell line.Outcomes revealed that MDR reversing effect was comparable to verapamil as positive control in vitro.Treatment of TPA-induced tumor promotion with 3-oxo-6β-hydroxy-β-amyrin led to reduction in the applied anti-tumor promotion experiment.The chemo-preventive effect of 3-oxo-6β-hydroxy-β-amyrin was comparable to curcumin as positive control based on the reduction of immediate early tumor antigen expression.Molecular docking by applying Autodock Vina 1 and i-GEMDOCK v 2.1 tools indicated that compound 1 gives good docking results,as determined by their fitness score and specificity.Moreover,results showed that compound 1 isolated from Pistacia integerrima precisely attached to a region where co-crystallized ligand for receptor previously existed.Our findings may explain the use of Pistacia integerrima plant extracts as an anticancer agent in folk medicine.

关 键 词：Pistacia integerrima Anti-tumor properties X-ray crystallography POM Molecular docking 

分 类 号：R73[医药卫生—肿瘤]