Long intergenic noncoding RNAs differentially expressed in Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells  

作　　者：JINGPENG ZHOU XIAOGUANG JI YUHAO WANG XIAOLONG WANG YONGJIANG MAO ZHANGPING YANG 

机构地区：[1]College of Animal Science and Technology,Yangzhou University,Yangzhou,225009,China [2]Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education,Yangzhou University,Yangzhou,225009,China [3]Hubei Blue Valley Microbial Technology Co.,Ltd.,Yichang,44300,China

出　　处：《BIOCELL》2021年第4期1033-1044,共12页生物细胞（英文）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31872324,31802035,and 31601915);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(18KJA230003);Six Talent Peaks Project in Jiangsu Province(NY-093);the China Postdoctoral Science Foundation(Grant Nos.2017M621841 and 2019T120472).

摘　　要：Cow mastitis is the most common disease that affects the dairy farming industry and causes serious harm to dairy cows and humans,and Staphylococcus(S.)aureus is one of the main pathogens that cause mastitis in dairy cows.In this study,a mastitis model was established through the infection of bovine mammary epithelial cells(BMECs)with S.aureus(bacterial concentration of 1×10^(9)/mL),and these cells and a blank group(untreated)were analyzed by flow cytometry(10000 cells,200 cells collected per second),hematoxylin and eosin(H&E)staining and immunohistochemistry.In addition,the lncRNAs(long non-coding RNAs)in the normal and S.aureus-infected BMEC group were screened by second-generation sequencing.Flow cytometry,H&E staining,and immunohistochemistry assays were performed to verify the successful construction of an S.aureus infection model in BMECs.A close relationship was found between the differential expression of lncRNAs and S.aureus mastitis.The total original sequencing reads were 627.13 M,and the average reads from each sample were approximately 104.52 M.After removing the unwanted reads,the total clean reads were 606.43 M,and the average reads from each sample were approximately 101.07 M.After S.aureus infection,30 lncRNAs were differentially expressed,and these included 21 upregulated and nine down-regulated lncRNAs.This research will not only expand our understanding of the lncRNA map in dairy cows but also help us hypothesize the function of lncRNAs in the genome and identify novel molecular markers of mastitis.

关 键 词：MASTITIS Staphylococcus aureus Bovine mammary epithelial cells lncRNA 

分 类 号：R73[医药卫生—肿瘤]