非转移性黑色素瘤糖蛋白B对脑缺血再灌注小鼠的神经保护作用  

Neuroprotective effects of glycoprotein nonmetastatic melanoma protein B on mice with cerebral ischemia reperfusion

作　　者：卢晓微 周智鹏[1] 张辉阳[1] 曾阳东[1] 成戈[1] 蒋宇 苏梦瑶 LU Xiao-wei;ZHOU Zhi-peng;ZHANG Hui-yang;ZENG Yang-dong;CHENG Ge;JIANG Yu;SU Meng-yao(Department of Radiology,Affiliated Hospital of Guilin Medical University,Guilin 541001,China)

机构地区：[1]桂林医学院附属医院放射科,广西桂林市541001

出　　处：《广西医学》2022年第6期602-605,共4页Guangxi Medical Journal

基　　金：国家自然科学基金(81760219);广西自然科学基金(2017GXNSFAA198058)。

摘　　要：目的探讨非转移性黑色素瘤糖蛋白B(GPNMB)对脑缺血再灌注小鼠的神经保护作用。方法将20只雄性C57小鼠随机分为磷酸缓冲盐溶液(PBS)组和GPNMB组,每组10只。采用线栓法栓塞大脑中动脉建立脑缺血小鼠模型,栓塞2 h后再灌注,GPNMB组经栓塞侧颈内动脉内注射4μL的50 ng/μL GPNMB,PBS组经颈内动脉给予注射等量的PBS。给予小鼠药物干预并再灌注24 h后处死,处死前进行行为学缺陷评分,制作脑组织切片,计算梗死体积。结果GPNMB组的脑梗死体积小于PBS组,栓塞再灌注后2 h、给药干预再灌注后24 h的行为学缺陷评分均低于PBS组(均P<0.05)。结论GPNMB可以减小脑缺血再灌注小鼠的脑梗死体积,并改善其梗死后行为学缺陷。Objective To explore the neuroprotective effects of glycoprotein nonmetastatic melanoma protein B(GPNMB)on mice with cerebral ischemia reperfusion.Methods Twenty male C57 mice were randomly divided into phosphate buffered saline(PBS)group and GPNMB group,with 10 mice in each group.The cerebral ischemia rat model was established by embolizing the middle cerebral artery using the suturing method.Two hours after the embolization followed reperfusion,the GPNMB group was injected with 4μL of 50 ng/μL GPNMB through the internal carotid artery on the embolic side,and the PBS group was injected with the same amount of PBS through the internal carotid artery.The mice were sacrificed after drug intervention and 24-hour reperfusion,and they were scored for behavioral defects before death.Mice′s brain tissue slices were prepared,and the infarcted volume was calculated.Results The GPNMB group exhibited a smaller volume of cerebral infarction as well as lower scores for behavioral defects after the 2-hour reperfusion following the embolization,and after the 24-hour reperfusion following the drug intervention,as compared with the PBS group(all P<0.05).Conclusion GPNMB can reduce the volume of cerebral infarction and improve the post-infarction behavioral defects in mice with cerebral ischemia reperfusion.

关 键 词：缺血再灌注 非转移性黑色素瘤糖蛋白B 行为学缺陷 脑梗死 小鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]