木薯块根不同发育期β-胡萝卜素和蛋白质积累变化及其有色体蛋白质组学研究  

Changes ofβ-carotene and Protein Accumulation and Proteomic Analysis of Chromoplasts in Different Developmental Stages of Cassava Tuberous Roots

作　　者：周锴 安飞飞[2] 李开绵[2] 陈松笔[2] ZHOU Kai;AN Feifei;LI Kaimian;CHEN Songbi(College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture and Rural Affairs for Germplasm Resources Conservation Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学热带作物学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所/农业农村部木薯种质资源保护与利用重点实验室,海南海口571101

出　　处：《热带作物学报》2022年第5期871-881,共11页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金面上项目(No.31871687)。

摘　　要：木薯是世界重要的粮食作物,在我国发展潜力巨大。蛋白质和类胡萝卜素是重要的营养品质特性,但木薯蛋白质含量低,提高木薯块根类胡萝卜素和蛋白质含量,提高其营养成分,已成为木薯选育种的重要目标。本研究以蛋黄木薯(‘SC9’)、紫叶黄心木薯(‘BGM019’)和白心面包木薯(‘SC101’)3个木薯品种为试验材料,开展黄心和白心木薯块根营养品质特性的研究,分析其不同发育期类胡萝卜素和蛋白质含量的变化,同时利用双向电泳和质谱法开展木薯块根有色体亚细胞结构蛋白质组的研究,揭示其蛋白质含量提高与类胡萝卜素积累的关联性。结果表明:3个木薯品种块根β-胡萝卜素含量在块根形成期、膨大期和成熟期差异显著(P<0.05),‘BGM019’和‘SC9’蛋白质含量在同一生长发育时期差异不显著(P>0.05),但显著高于‘SC101’(P<0.05)。随着木薯块根发育成熟,其类胡萝卜素和蛋白质含量均呈上升趋势,且二者表现为正相关。以膨大期为对照,在‘SC9’成熟期块根有色体中检测到36个差异表达蛋白质,其中26个上调表达,10个下调表达;‘BGM019’中检测到49个差异表达蛋白质,其中28个上调表达,21个下调表达;‘SC101’检测到38个差异蛋白质,其中20个上调表达,18个下调表达。通过对上述差异蛋白质的功能分析,发现它们均参与抗氧化、碳代谢和能量代谢、信号转导等多个代谢过程,说明木薯块根类胡萝卜素积累是由多个代谢途径协同作用的结果。对比分析3个木薯品种块根有色体亚细胞结构的差异蛋白质,发现20个差异表达蛋白质在其中2个品种或3个品种间出现相同的表达趋势。它们主要涉及到碳代谢及能量代谢、抗氧化、细胞骨架蛋白、无机离子运输及代谢、分子伴侣和蛋白质合成等代谢途径,其中与抗氧化相关的蛋白质占20%。构建蛋白质生物调控网络揭示块根有色体蛋白质�Cassava is one of the most important crops in the world and has a great potential as food crop in China.Protein and carotenoids are important nutritional quality characteristics.The content of protein in the cassava tuberous root is low.Hence,to improve the carotenoid and protein content and the value nourishment of the tuberous root is an important goal for cassava breeding.In the present study,three cassava varieties including yellow cassava‘SC9’,‘BGM019’and white cassava‘SC101’were selected to analyze the nutritional quality characteristics,and measure the change ofβ-carotene and protein content at different developmental stages of cassava tuberous roots.Additionally,2-DE in combination with mass spectrometry were used to analyze the proteome profile of the chromoplast in order to understand the coupling mechanism of increasing the protein content related with carotenoid accumulation.The results showed that there were significant differences(P<0.05)between the genotypes inβ-carotene content in the periods of formation,enlargement and maturation of cassava tuberous roots.There were no significant differences(P>0.05)between‘SC9’and‘BGM019’regarding the protein content of tuberous roots,however,the protein contents in both yellow cassava genotypes weresignificantly higher than that of‘SC101’(P<0.05).A positive correlation between the carotenoid and protein content was observed during the development of the tubers in the genotypes.The tuberous-root chromoplasts of enlargement period were used as references,proteome profile of tuberous-root chromoplast in maturation period revealed a total of 36 protein spots in‘SC9’,in which 26 were classified as up-regulated and 10down-regulated,while in‘BGM019’,49 differential proteins were identified with 28 up-regulated spots and 21down-regulated,and in‘SC101’38 differential proteins identified,in which 20 were up-regulated and 18 were down-regulated.Most of the identified differential proteins were found to be involved in carbon and energ

关 键 词：木薯块根 蛋白质含量 β-胡萝卜素含量 有色体 蛋白质组学 

分 类 号：S533[农业科学—作物学]