山东省8例尖锐湿疣患者人乳头瘤病毒6型全基因组序列分析  

Whole genome sequencing of human papillomavirus type 6 from eight condyloma acuminatum patients in Shandong province

作　　者：张翠 王素婷[2] 林小娟[2] 纪峰[2] 寇增强[2] 徐爱强 陶泽新[2] 宋艳艳[1] Zhang Cui;Wang Suting;Lin Xiaojuan;Ji Feng;Kou Zengqiang;Xu Aiqiang;Tao Zexin;Song Yanyan(School of Public Health,Cheeloo College of Medicine y Shandong University,Jinan 250012,Shandong y China;Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention,Shandong Provincial Center for Disease Control and Prevention,Jinan 250014,Shandong,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院,山东济南250012 [2]山东省疾病预防控制中心山东省传染病预防控制重点实验室,山东济南250014

出　　处：《中国疫苗和免疫》2022年第2期189-194,共6页Chinese Journal of Vaccines and Immunization

基　　金：泰山学者工程专项经费资助(ts.201511105);泰山学者青年专家计划项目(tsqn.202103187)。

摘　　要：目的分析山东省人乳头瘤病毒6型(Human papillomavirus type 6,HPV6)全基因组序列谱系和变异。方法于2019年在山东省3家哨点医院采集尖锐湿疣患者肛门拭子,提取HPV6 DNA进行PCR扩增和测序,对获得的HPV6全基因组序列进行同源性、系统进化和变异分析。结果本研究获得8条HPV6全基因组序列,其中5条属于A谱系,3条属于B1谱系;8条HPV6序列共发生165个核苷酸变异,错义突变率为22.4%(37/165);8条序列的核苷酸同源性为98.48%-99.99%;A谱系和B1谱系的核苷酸变异数分别为109个、56个,错义突变率分别为14.7%(16/109)、37.5%(21/56);两个谱系中E6、E7和E4编码区未发生或极少发生突变。结论山东省尖锐湿疣患者HPV6流行株为A谱系和B1谱系,与全球HPV6流行谱系分布一致;A谱系比B1谱系更保守。Objective To analyze lineages and nucleotide mutations of whole genome sequences of human papillomavirus type 6(HPV6) in Shandong province.Methods We collected anogenital swabs from condyloma acuminata patients in three sentinel hospitals of Shandong in 2019 and extracted HPV6 DNA for PCR amplification and sequencing. We analyzed homologies, phylogenies, and mutations of HPV6 whole genome sequences.Results Eight HPV6 whole genome sequences were obtained in the study;5 belonged to the A lineage and 3 belonged to the B1 lineage. A total of 165 nucleotide mutations occurred in the 8 HPV6 sequences, with the sense mutation rate of 22.4%(37/165). Nucleotide homologies of the 8 sequences were 98.48%-99.99%;109 and 56 nucleotide mutations occurred in the A and B1 lineages, respectively, with sense mutation rates of 14.7%(16/109) and 37.5%(21/56). No or very few mutations occurred in the coding regions of E6, E7, and E4 in the two lineages.Conclusions HPV6 strains obtained from condyloma acuminata patients of Shandong were A and B1 lineages, the same as the global HPV6 lineages. Lineage A was more conserved than lineage B1.

关 键 词：尖锐湿疣 人乳头瘤病毒6型 全基因组测序 同源性 变异 

分 类 号：R373.2[医药卫生—病原生物学]