1株异养硝化-好氧反硝化神户肠杆菌的鉴定及脱氮特性  

Identification of a Heterotrophic Nitrification-Aerobic Denitrifying Bacterium and Its Removal Characteristics of Nitrogen

作　　者：胡丹 何富强 杜全能 王刚 兰时乐[1] HU Dan;HE Fu-qiang;DU Quan-neng(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128;Hunan Institute of Microbiology,Changsha,Hunan 410009;Haida Research Institute of Guangdong Haida Group Co.,Ltd,Guangzhou,Guangdong 511400)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南省微生物研究院,湖南长沙410009 [3]广东海大集团股份有限公司海大研究院,广东广州511400 [4]湖南华君农业科技有限公司,湖南益阳413000

出　　处：《安徽农业科学》2022年第10期70-74,共5页Journal of Anhui Agricultural Sciences

基　　金：湖南省重点研发项目(2016NK2103);湖南创新型省份建设经费资助项目(2020NK2029)。

摘　　要：[目的]鉴定1株异养硝化-好氧反硝化神户肠杆菌,明确其脱氮特性。[方法]从养殖池塘底泥中筛选到1株异养硝化-好氧反硝化菌HD-NAH,经形态学观察、生理生化试验以及16S rDNA序列分析,鉴定为神户肠杆菌(Enterobacter kobei)HD-NAH,并研究其脱氮特性。[结果]该菌在以柠檬酸钠为碳源,C/N为18,初始pH为7,温度为27℃,转速为190 r/min时,24 h亚硝氮(NO_(2)^(-)-N)和总氮(TN)降解率分别为99.98%和89.37%,具有较高的降解效率。菌株在初始pH为7~10,温度为27~37℃,转速为130~210 r/min时,对NO_(2)^(-)-N和TN的降解率均较高,表明该菌株的环境适应性较强。在不同氮源条件下,菌株HD-NAH对氮的去除存在差异,其对TN去除率表现为NO_(2)^(-)-N>NH_(4)^(+)-N+NO_(2)^(-)-N>NH_(4)^(+)-N+NO_(3)^(-)-N>NH_(4)^(+)-N>NO_(3)^(-)-N,还存在一定短程异养硝化-好氧反硝化过程。[结论]菌株HD-NAH良好的脱氮特性可为养殖废水除氮提供可选择材料。[Objective]To identify a heterotrophic nitrification-aerobic denitrifying bacterium,and clarify its denitrification characteristics.[Method]A heterotrophic nitrification-aerobic denitrifying bacterium was screened from the sludge of aquaculture pond,was named as HD-NAH.The strain was identified as Enterobacter kobei by morphological observation,physiological and biochemical tests,and 16S rDNA sequence analysis.The removal characteristics of nitrite nitrogen and total nitrogen by HD-NAH were studied.[Result]The results showed that degradation rate of nitrite nitrogen and total nitrogen by strain HD-NAH were 99.98%and 89.37%respectively,under the conditions of trisodium citrate as the sole carbon source,C/N 18,pH 7,appropriate culture temperature 27℃and shaking speed 190 r/min.The strain HD-NAH had preferable degradation rate of nitrite nitrogen and total nitrogen under pH 7-10,temperature 27-37℃,and shaking speed 130-210 r/min,it means that strain HD-NAH has strong environmental adaptability.The degradation rate of nitrite nitrogen and total nitrogen were measured under different nitrogen source,the rate was arranged as:NO_(2)^(-)-N>NH_(4)^(+)-N+NO_(2)^(-)-N>NH_(4)^(+)-N+NO_(3)^(-)-N>NH_(4)^(+)-N>NO_(3)^(-)-N.The strain showed a certain short-range heterotrophic nitrification-aerobic denitrification process.[Conclusion]It provided an alternative material for the denitrification of aquaculture wastewater basing on good denitrification characteristics of the strain HD-NAH.

关 键 词：神户肠杆菌HD-NAH 异养硝化 好氧反硝化 脱氮特性 

分 类 号：X172[环境科学与工程—环境科学]