Fonsecaea monophora色素株促进巨噬细胞凋亡的研究  

Melanized meristematic mutant of Fonsecaea monophora promotes macrophage apoptosis

作　　者：李敏英 黄欢 刘红芳 曾维英 席丽艳 LI Minying;HUANG Huan;LIU Hongfang;ZENG Weiying;XI Liyan(Dermatology Hospital,Southern Medical University,Guangzhou 510091,China;Sun Yat-sen Memorial Hospital of Sun Yat-sen University,Guangzhou 510120,China)

机构地区：[1]南方医科大学皮肤病医院,广东广州510091 [2]中山大学孙逸仙纪念医院,广东广州510120

出　　处：《皮肤性病诊疗学杂志》2022年第2期124-129,共6页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：国家自然科学基金(81873960,81601746);广东省医学科学技术研究基金(A2019345)。

摘　　要：目的探讨Fonsecaea monophora(F.monophora)色素株对巨噬细胞RAW264.7凋亡的影响。方法将巨噬细胞分别与F.monophora色素株和白化株进行共培养,设置PBS对照组,Western blot检测PARP-1的表达;实时定量PCR检测各组中BCL-2、BAX的表达;Annexin V FITC荧光染色检测凋亡。结果孢子与巨噬细胞共培养后,F.monophora色素株较白化株贴壁的巨噬细胞明显减少,并且能明显切割PARP-1,下调BCL-2/BAX表达的比值,上调Annexin V FITC的阳性细胞数量。结论F.monophora色素株可能通过促进巨噬细胞凋亡,进而抵抗巨噬细胞的免疫杀伤作用;而白化株不能明显促使巨噬细胞的凋亡。Objective To explore the effect of Fonsecaea monophora(F.monophora)pigment strain melanin on the apoptosis of macrophages RAW264.7.Methods Macrophages were co-cultured with PBS,F.monophora pigment strain and albino strain,Western blot was used to detect the expression of PARP-1;and real-time quantitative PCR was used to detect the expression of BCL-2 and BAX in each group.Annexin V FITC fluorescent staining was used to detect apoptosis.Results After co-cultured with macrophages RAW264.7,the pigmented strain was more capable of cutting PARP-1,down-regulating the ratio of BCL-2/BAX expression,and up-regulating Annexin V FITC positive cells than the albino strain.Conclusion The F.monophora pigment strain may resist the immune killing effect of macrophages by promoting the apoptosis of macrophages,while the albino strains cannot obviously promote the apoptosis of macrophages.

关 键 词：Fonsecaea monophora色素株 巨噬细胞 凋亡 

分 类 号：R73[医药卫生—肿瘤]