三阴性乳腺癌中着丝粒蛋白K表达与上皮间质转化的关系及其临床意义  

Correlation between expression of centromere protein K and epithelial-mesenchymal transition in triple-negative breast cancer and its clinical significance

作　　者：靳继海[1] 蔺广荣 张红婕 王晓霞 Jin Jihai;Lin Guangrong;Zhang Hongjie;Wang Xiaoxia(Department of Breast Surgery,Binzhou People's Hospital Affiliated to Shandong First Medical University,Binzhou 256610,China;Department of Thyroid Surgery,Binzhou People's Hospital Affiliated to Shandong First Medical University,Binzhou 256610,China;Department of Operating Room,Binzhou People's Hospital Affiliated to Shandong First Medical University,Binzhou 256610,China)

机构地区：[1]山东第一医科大学附属滨州市人民医院乳腺外科,滨州256610 [2]山东第一医科大学附属滨州市人民医院甲状腺外科,滨州256610 [3]山东第一医科大学附属滨州市人民医院手术室,滨州256610

出　　处：《肿瘤研究与临床》2022年第3期176-179,共4页Cancer Research and Clinic

基　　金：山东省医药卫生科技发展计划(202004011168)。

摘　　要：目的探讨着丝粒蛋白K(CENPK)在三阴性乳腺癌(TNBC)中的表达与上皮间质转化(EMT)的关系及其临床意义。方法采用免疫组织化学SABC法检测2015年1月至2018年12月山东第一医科大学附属滨州市人民医院69例TNBC患者肿瘤组织和相应癌旁组织中CENPK及EMT相关蛋白(E-cadherin、Vimentin、N-cadherin)的表达,分析CENPK表达与患者临床病理特征及EMT相关蛋白表达的相关性。结果CENPK在TNBC及癌旁组织中的阳性表达率分别为72.46%(50/69)、14.49%(10/69),差异有统计学意义(χ^(2)=47.18,P<0.001)。CENPK在TNBC中的表达与病理分级、临床分期及淋巴结转移均相关(均P<0.05)。CENPK在TNBC中的表达与E-cadherin表达呈负相关(r=-0.447,P<0.01),与Vimentin、N-cadherin表达均呈正相关(r值分别为0.503、0.415,均P<0.01)。CENPK高表达组中位总生存时间为24个月(95%CI 10~39个月),低表达组为42个月(95%CI 19~50个月),两组总生存差异有统计学意义(χ^(2)=7.36,P=0.016)。结论CENPK可能通过EMT参与TNBC的发生、发展,且CENPK表达可能与患者预后有关。Objective To investigate the correlation of centromere protein K(CENPK)expression in triple-negative breast cancer(TNBC)with epithelial-mesenchymal transformation(EMT)and its clinical significance.Methods Immunohisochemical SABC method was used to detect the expressions of CENPK and EMT-related proteins(E-cadherin,Vimentin and N-cadherin)in 69 specimens of TNBC and cancer-adjacent tissues collected from patients in Binzhou People's Hospital Affiliated to Shandong First Medical University from January 2015 to December 2018.The correlations of CENPK expression with clinicopathological characteristics of patients and expressions of EMT-related proteins were analyzed.Results The positive expression rates of CENPK in TNBC and cancer-adjacent tissues were 72.46%(50/69)and 14.49%(10/69),respectively,and the difference was statistically significant(χ^(2)=47.18,P<0.001).The CENPK expression in TNBC was related to pathological grade,clinical stage and lymphatic metastasis(all P<0.05).The CENPK expression in TNBC was negatively correlated with E-cadherin expression(r=-0.447,P<0.01),but positively correlated with Vimentin and N-cadherin expressions(r values were 0.503 and 0.415,both P<0.01).The median overall survival time of CENPK high-expression group was 24 months(95%CI 10-39 months),while that of CENPK low-expression group was 42 months(95%CI 19-50 months).The difference in overall survival between the two groups was statistically significant(χ^(2)=7.36,P=0.016).Conclusions CENPK may be involved in the occurrence and development of TNBC through EMT,and the expression of CENPK may be related to the prognosis of patients.

关 键 词：乳腺肿瘤 免疫组织化学 着丝粒蛋白K 上皮细胞-间充质细胞转换 预后 

分 类 号：R737.9[医药卫生—肿瘤]