母子分离对青年CD-1雄鼠认知功能及海马BDNF介导的LTP的影响  

Effects of maternal separation on cognitive function and BDNF-induced LTP of hippocampus in young CD-1 male mice

作　　者：王亚涛 张月明 韦琪瑶 吴永芳 陈贵海[1] Wang Yatao;Zhang Yueming;Wei Qiyao;Wu Yongfang;Chen Guihai(Dept of Neurology(Dept of Sleep Disorders),Chaohu Hospital of Anhui Medical University,Hefei 238000;Dept of Neurology,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学附属巢湖医院神经内科(睡眠障碍科),巢湖238000 [2]安徽医科大学第二附属医院神经内科,合肥230601

出　　处：《安徽医科大学学报》2022年第5期720-724,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81671316)。

摘　　要：目的探究海马脑源性神经营养因子(BDNF)介导的长时程增强(LTP)是否参与母子分离(MS)导致子雄鼠青年期认知功能受损的过程。方法新生CD-1雄鼠随机分为母子分离组(MS组)和对照组(CON组),其中,MS组小鼠从出生后第4~21天每日与母鼠分开3 h,CON组不进行任何干预。两组小鼠在3月龄时用Morris水迷宫实验检测子鼠空间学习记忆能力,Western blot和RT-PCR检测子鼠海马BDNF表达水平,电生理技术检测海马CA3-CA1通路的LTP。结果与对照组相比,MS组学习期潜伏期及游泳路程均延长(P<0.01),记忆期靶象限时间及路程百分比均减少(P<0.05)。同时MS组海马的BDNF表达水平下降(P<0.05)和CA3-CA1通路的LTP受损(P<0.01)。结论一定强度的母子分离操作可以损害青年CD-1雄鼠的认知功能,且可能与海马区BDNF表达水平下降及LTP受损有关。Objective To explore whether long-term potentiation(LTP)induced by brain-derived neurotrophic factor(BDNF)of hippocampus is involved in the process of maternal separation(MS)leading to impaired cognitive function of offspring in adolescence.Methods The newborn CD-1 mice were randomly divided into maternal separation group(MS group)and control group(CON group).Mice in MS group were separated from the mother mice for 3 h every day from postnatal day 4 to 21 while no intervention was taken in the CON group.The spatial learning and memory ability was assessed using Morris water maze at the age of 3 months.Western blot and real-time quantitative PCR were used to detect the levels of BDNF and BDNF mRNA in the hippocampus.LTP of the hippocampal CA3-CA1 neural pathway was recorded using electrophysiological techniques.Results Compared with CON group,the latency and distance of Morris water maze in maternal separation group were significantly longer(P<0.01).The percentage of time and distance in target quadrant during the memory phase in MS group were obviously lower than those in control group(P<0.05).The results of WB and Real-time quantitative PCR in MS group showed that the levels of BDNF and BDNF mRNA in MS group apparently decreased(P<0.05).Compared with CON group,MS group showed a significantly lower LTP in CA3-CA1 neural pathway(P<0.01).Conclusion The certain intensity of maternal separation can impair learning and memory function in young CD-1 male mice,which may be associated with decreased expression of BDNF and impaired LTP in the hippocampus.

关 键 词：母子分离 学习记忆 脑源性神经营养因子 长时程增强 

分 类 号：R741.02[医药卫生—神经病学与精神病学]