SD大鼠外伤性特纳牙模型建立及微观结构观察  

Model establishment and microstructure observation of Turner′s tooth caused by trauma in SD rat

作　　者：高黎[1] 郭姗 李艺博 卢丽雯 张彦喜 Gao Li;Guo Shan;Li Yibo;Lu Liwen;Zhang Yanxi(Dept of Pedodontics,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052;Dept of Oral Sugery,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院儿童口腔科,郑州450052 [2]郑州大学第一附属医院口腔颌面外科,郑州450052

出　　处：《安徽医科大学学报》2022年第5期731-735,共5页Acta Universitatis Medicinalis Anhui

基　　金：河南省科技厅科技攻关项目(编号:172102310487);河南省高等学校重点科研项目(编号:18A310037)。

摘　　要：目的建立SD大鼠外伤性特纳牙模型,使用扫描电子显微镜(SEM)观察其表面结构和进行能谱分析(EDS)。方法将40只1 d龄SD大鼠随机分为四组,每组10只。对照组:不做任何处理;实验组:分别垂直于大鼠下颌前牙区牙槽突加力,分5N力组、10N力组、15 N力组。受力面积均为2.5 mm×2.0 mm。30 d龄时处死大鼠,分别记录其下颌中切牙釉质情况,并通过SEM观察正常釉质、发育不全釉质微观结构及进行能谱分析。结果对照组牙齿牙釉质正常,均正常萌出;5N力组牙釉质发育不全率10%(釉质变色2颗),未萌0颗;10N力组牙釉质发育不全率80%(釉质变色12颗,釉质缺损4颗),未萌1颗;15N力组牙釉质发育不全率60%(釉质变色3颗,釉质缺损9颗),未萌7颗。10N力组与15N力组未萌牙差异有统计学意义(P<0.05),SEM下釉质发育不全部分区域釉质表面粗糙伴裂纹,部分区域凹凸不平。EDS结果显示釉质发育不全区钙磷含量较正常釉质低(P<0.05)。结论牙胚外伤可引起釉质发育不全和未萌牙,5 mm^(2)受力面施加10 N力适合建立大鼠外伤性特纳牙模型。外伤性特纳牙表面釉质粗糙,凹凸不平,钙磷含量减少。Objective To establish an experimental model of Turner′s tooth caused by trauma in SD rats and observe its surface by scanning electronic microscopes(SEM)and energy dispersive spectrometry(EDS)was performed.Methods 40 SD rats aged one day were randomly divided into four groups(n=10).The control group did nothing,The experimental group was exerted different perpendicular forces(per 5 mm^(2))on the mandibular anterior alveolar process,which was divided into 5N force group,10N force group and 15N force group.The SD rats were killed at 30 days old to observe the enamel development of their mandibular central incisors.Meanwhile,SEM and EDS were used to observe normal enamel area and enamel hypoplasia area.Results The control group:all teeth erupted;all enamels developed well.5N force group:all teeth erupted;the occurrence rate of enamel hypoplasia was 10%(2 teeth had enamel discoloration).10N force group:1 tooth unerupted;the occurrence rate of enamel hypoplasia was 80%(12 teeth had enamel discoloration and 4 had enamel defects).15N force group:7 teeth unerupted,the occurrence rate of enamel hypoplasia was 60%(3 teeth had enamel discoloration and 9 had enamel defects).There was a statistic difference in the number of unerupted teeth between group 10N force group and 15N force group(P<0.05).Under SEM,cracks and rough appeared on the surface of enamel.EDS showed that the Ca and P content in enamel hypoplasia was lower than that in the normal enamel area(P<0.05).Conclusion Tooth trauma can lead to enamel hypoplasia and unerupted teeth.The force of 10 N per 5 mm^(2) is better to establish an experimental model of Turner′s tooth caused by trauma in SD rats.The surface enamel of Turner′s tooth caused by trauma is rough,uneven and the content of calcium and phosphorus decreases.

关 键 词：SD大鼠 特纳牙 外伤 釉质发育不全 

分 类 号：R788.4[医药卫生—口腔医学] R788.2[医药卫生—临床医学]