Lnc-MEG3调控miR-10b-5p/CD82轴对垂体瘤细胞增殖、侵袭的影响  

Effects of lnc-MEG3 on the proliferation and invasion of pituitary tumor cells by regulating miR-10b-5p/CD82 axis

作　　者：郑锴 罗秀玲 张玮豪 刘宇利 李玉明 廖尚高 ZHENG Kai;LUO Xiuling;ZHANG Weihao;LIU Yuli;LI Yuming;LIAO Shanggao(Department of Neurosurgery,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China;不详)

机构地区：[1]贵州医科大学附属医院神经外科,贵阳550004 [2]贵州医科大学附属医院头颈肿瘤科,贵阳550004 [3]贵州医科大学药学院,贵阳550004

出　　处：《实用医学杂志》2022年第8期958-963,共6页The Journal of Practical Medicine

基　　金：贵州省高层次创新型人才资助项目(编号:黔科合平台人才[2020]6011)。

摘　　要：目的探究lnc-MEG3对垂体瘤细胞增殖、侵袭的调控机制。方法取垂体瘤HP75细胞分为对照组、si-NC组、si-MEG3组、pcDNA3.1-NC组、oe-MEG3组、oe-MEG3+mimic-NC组、oe-MEG3+miR-10b-5p mimic组。转染后,检测细胞中lnc-MEG3、miR-10b-5p和CD82 mRNA的表达水平、细胞增殖、迁移、侵袭能力和细胞中CD82、PCNA、E-cadherin、N-cadherin、MMP-9蛋白表达。结果Lnc-MEG3过表达可降低miR-10b-5p,上调CD82和E-cadherin水平,降低PCNA、N-cadherin、MMP-9水平,抑制垂体瘤细胞增殖、迁移和侵袭(P<0.05);上调miR-10b-5p可抑制CD82,减弱lnc-MEG3过表达对垂体瘤细胞增殖、侵袭和迁移的抑制作用。双荧光素酶报告基因检测结果显示,转染miR-10b-5p mimic后,细胞中MEG3-WT和CD82-WT的荧光素酶活性显著降低(P<0.05)。结论Lnc-MEG3过表达可能通过靶向下调miR-10b-5p,进而上调CD82的表达,抑制垂体瘤细胞增殖、迁移和侵袭。Objective To explore the regulation mechanism of lnc-MEG3 on the proliferation and invasion of pituitary tumor cells.Methods The purchased pituitary tumor HP75 cells were divided into control group,si-NC group,si-MEG3 silence group,pcDNA3.1-NC group,oe-MEG3 group,oe-MEG3+mimic-NC group and oe-MEG3+miR-10b-5p mimic group.After transfection,RT-qPCR was used to detect the expression levels of lnc-MEG3,miR-10b-5p and CD82 mRNA of cells in each group.CCK-8 method,scratch test and Transwell test were used to detect cell proliferation,migration and invasion abilities.Western blot was used to detect the protein expression of CD82,PCNA,E-cadherin,N-cadherin,MMP-9 of cells.Dual luciferase reporter gene was used to detect the targeting relationship between miR-10b-5p and lnc-MEG3,CD82.Results Overexpression of lnc-MEG3 significantly reduced the expression of miR-10b-5p,up-regulated CD82 and E-cadherin levels in cells,decreased PCNA,N-cadherin,and MMP-9 levels,and inhibited the proliferation and migration of pituitary tumor cells and invasion(P<0.05).Op-regulation of miR-10b-5p inhibited the expression of CD82,and weakened the inhibitory effect of lnc-MEG3 overexpression on the proliferation,invasion and migration of pituitary tumor cells.The results of dual luciferase reporter gene detection showed that after transfection of miR-10b-5p mimic,the luciferase activities of MEG3-WT and CD82-WT in the cells were significantly reduced(P<0.05).Conclusion The overexpression of lnc-MEG3 may inhibit the proliferation,migration and invasion of pituitary tumor cells by targeting to down-regulate miR-10b-5p and then up-regulating the expression of CD82.

关 键 词：垂体瘤 长链非编码RNA母系表达基因3 MicroRNA-10b-5p CD82 

分 类 号：R739.41[医药卫生—肿瘤]