耐低温菌株的鉴定、低温生长特征及耐低温机理初步研究  

Identification,low temperature growth characteristics and tolerance mechanism of low temperature-resistant strain

作　　者：杨洋 包昌杰 李柯欣 潘莹 王馨钰 方国权 陈欢[1] 张斯童[1] 陈光[1] 王刚[1] YANG Yang;BAO Changjie;LI Kexin;PAN Ying;WANG Xinyu;FANG Guoquan;CHEN Huan;ZHANG Sitong;CHEN Guang;WANG Gang(Key Laboratory of Straw Biology and Utilization,Ministry of Education,College of Life Science,Jilin Agricultural University,Changchun 130118,China;College of Life Science,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院秸秆生物学与利用教育部重点实验室,吉林长春130118 [2]吉林农业大学生命科学学院,吉林长春130118

出　　处：《中国酿造》2022年第5期47-51,共5页China Brewing

基　　金：吉林省科技厅重点研发计划(202000402097NC)。

摘　　要：该研究以分离自长白山常年积雪覆盖的苔原带土样的耐低温菌株7a-3为研究对象,采用形态观察、生理生化试验及分子生物学技术对其进行菌种鉴定,并对其生长特性及耐低温机制进行初步研究。结果表明,菌株7a-3被鉴定为莓实假单胞菌(Pseudomonas fragi),其生长温度范围为4~33℃,最适生长温度为30℃,表面活性剂不能促进该菌株在37℃条件下生长,但其自身低温发酵代谢物可促进该菌在37℃条件下生长。相对于30℃,4℃条件下,菌株7a-3细胞膜的脂肪酸种类由长链脂肪酸向短链脂肪酸转化,不饱和脂肪酸的相对含量增加,且单不饱和脂肪酸相对含量的变化较大,细胞膜较为粗糙,初步推测其耐低温能力与细胞膜不饱和脂肪酸含量增加导致细胞膜流动性增强相关。In this study,a low temperature-resistant strain 7a-3,isolated from tundra soil sample covered with perennial snow from Changbai Mountains,was identified by morphological observation,physiological and biochemical tests,and molecular biological techniques,and its growth characteristics and mechanism of low-temperature tolerance were investigated.The results showed that the strain 7a-3 was identified as Pseudomonas fragi,the growth temperature ranged from 4-33℃,the optimal growth temperature was 30℃,the surfactants could not promote the growth of the strain at 37℃,but its own low temperature fermentation metabolites could promote the growth of the strain at 37℃.Relative to the condition of 30℃,under the conditions of 4℃,the fatty acid species of the cell membrane of strain 7a-3 were converted from long-chain fatty acids to short chain fatty acids,the relative content of unsaturated fatty acids increased,the relative content of monounsaturated fatty acids changed greatly,and the cell membrane was rough.It was preliminarily speculated that the low temperature tolerance was related to the enhancement of cell membrane fluidity caused by the increase of unsaturated fatty acid content.

关 键 词：莓实假单胞菌 鉴定 生长特性 低温 机理 

分 类 号：Q180.61[生物学—普通生物学]