氧化硫硫杆菌固定化菌球制备及其耦合填料去除H_(2)S  

The preparation of Acidithiobacillus thiooxidans immobilized pellet and its coupling removal of H_(2)S with packing material

作　　者：石超宏 卢钰升 顾文杰 解开治 徐培智 王丹 彭焕龙 李雅莹 孙丽丽 SHI Chao-hong;LU Yu-sheng;GU Wen-jie;XIE Kai-zhi;XU Pei-zhi;WANG Dan;PENG Huan-long;LI Ya-ying;SUN Li-li(Key Laboratory of Plant Nutrition and Fertilizer in South Region,Ministry of Agriculture,Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation,Guangdong Engineering Research Center of Soil Microbes and Cultivated Land Conservation,Institute of Agricultural Resources and Environment,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Maoming 525000,China)

机构地区：[1]广东省农业科学院农业资源与环境研究所,农业部南方植物营养与肥料重点实验室,广东省养分资源循环利用与耕地保育重点实验室,广东省土壤微生物与耕地保育工程技术研究中心,广东广州510640 [2]岭南现代农业科学与技术广东省实验室茂名分中心,广东茂名525000

出　　处：《中国环境科学》2022年第5期2331-2338,共8页China Environmental Science

基　　金：广东省重点领域研发计划项目(2020B0202090004);广东省农业科学院院长基金资助项目(201934);广东省农业科学院创新基金资助项目(202226);广东省农业科学院农业优势产业学科团队建设项目(202121TD);广东省基础与应用基础研究基金资助项目(2021A1515011211);广东省高端外国专家项目(粤科函资字〔2019〕1538号);广东省现代农业产业技术体系创新团队项目(2021KJ118);茂名实验室自主科研项目(2021ZZ003);广州市科技计划项目(202002020075)。

摘　　要：以海藻酸钠(SA)为包埋载体,对氧化硫硫杆菌(Acidithiobacillus thiooxidans)进行固定化制备菌球,优化菌球制备条件,在生物滤塔内验证其对H_(2)S的去除能力.以前期驯化获得的富含硫系恶臭降解微生物菌群的污泥为菌源进行生物滤塔填料筛选,耦合A.thiooxidans菌球和填料进行生物除臭.结果表明,优选的固定化条件为:SA浓度3.0%､吸附剂CNT､A.thiooxidans菌悬液与混合液比例20%､CaCl_(2)浓度4.0%､改性剂己二胺溶液,获得的菌球机械强度､传质性能､硫氧化能力最好.将A.thiooxidans菌球填装于生物滤塔,H_(2)S最大去除率和去除能力为70%和1.06g H_(2)S/m^(3)·h.以混合挂膜方式进行填料挂膜后,在聚氨酯泡沫､活性碳布和陶粒中优选出最佳填料活性碳布,获得H_(2)S最大去除率和去除能力为88%和0.84g H_(2)S/m^(3)·h.以混合填装方式将A.thiooxidans菌球与活性碳布填装于生物滤塔,获得H_(2)S最大去除率和去除能力为86%和1.00g H_(2)S/m^(3)·h.Acidithiobacillus thiooxidans was immobilized to prepare bacterial pellet using sodium alginate(SA)as the carrier.The microbial immobilization process was optimized and the removal capacity of the bacterial pellet to H_(2)S was examined in a biofilter.Meanwhile,acclimated activated sludge containing sulfur malodorous eliminating microbial consortium obtained previously was employed as bacterial source to screen packing media in biofilter.Lastly,A.thiooxidans immobilized pellet and the screened packing media were coupled to biotrickling filtration deodorization.The results showed that the optimal conditions for microbial immobilization process were 3.0%of SA,adding adsorbent carbon nanotubes(CNT)into SA solution,keeping the ratio of A.thiooxidans bacterial suspension solution to the mixture solution at 20%,4.0%of CaCl_(2),suspending immobilized pellet into hexanediamine(HDA)solution for surface modification.Filling A.thiooxidans immobilized pellet into biofilter to eliminate H_(2)S,and the removal efficiency and elimination capacity of H_(2)S reached 70%and 1.06g H_(2)S/m^(3)·h.Activated carbon cloth was screened as the best packing media among polyurethane foam,activated carbon cloth and ceramsite,and the removal efficiency and elimination capacity of H_(2)S reached 88%and 0.84g H_(2)S/m^(3)·h in this case.Filling A.thiooxidans immobilized pellet and activated carbon cloth into biofilter with mixing pattern to eliminate H_(2)S,the removal efficiency and elimination capacity of H_(2)S reached 86%and 1.00g H_(2)S/m^(3)·h.

关 键 词：微生物固定化 氧化硫硫杆菌 H_(2)S 生物滴滤 

分 类 号：X172[环境科学与工程—环境科学] X701