红米‘rm257’种皮颜色遗传模式分析及Rc基因功能标记开发  

Genetic analysis of pericarp color and functional markers development for Rc gene of red rice germplasm‘rm257’

作　　者：李霞[1] 杜娟[1] 杨晓梦[1] 普晓英[1] 杨加珍[1] 杨涛[1] 李玉萍 曾亚文[1] LI Xia;DU Juan;YANG Xiao-meng;PU Xiao-ying;YANG Jia-zhen;YANG Tao;LI Yu-ping;ZENG Ya-wen(Biotechnology and Genetic Resources Institute,Yunnan Academy of Agricultural Sciences/Agricultural Biotechnology Key Laboratory of Yunnan Province,Kunming 650205,China;Yuxi Agriculture Vocation-Technical College,Yuxi,Yunnan 653106,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室,昆明650205 [2]玉溪农业职业技术学院,云南玉溪653106

出　　处：《西南农业学报》2022年第5期1010-1015,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(31760376);云南省基础研究计划项目(2018FB059)。

摘　　要：【目的】红米种质‘rm257’在籽粒总黄酮和抗性淀粉含量方面表现突出,分析该材料种皮颜色遗传模式,将为其在实际育种应用中奠定基础。同时探究‘rm257’种皮颜色性状与Rc之间的关系并开发对应功能标记,为云南红米品种的辅助选育提供技术支持,提高育种效率。【方法】利用红米种质‘rm257’与9份云南地方白米品种构建测交组合及F_(2)分离群体,通过统计测交当代和对应F_(2)分离群体种皮颜色和分离比,推测其遗传模式;同时依据已报导Rc基因缺失变异位点序列开发功能标记RCGX,对9个F_(2)分离群体单株进行基因型鉴定,并将基因型与对应单株表型进行成对分析,进而判断红米种质‘rm257’种皮颜色与Rc基因的关系。同时利用云南96份地方种对功能标记RCGX的通用性进行检验,评估该功能标记在红米种皮颜色判定中的准确性。【结果】遗传模式分析表明,红米种质‘rm257’的种皮颜色在7个测交组合中表现为受1对显性基因控制,在2个测交组合中表现为受2个基因位点的控制与影响。这与前人研究报导的红米种皮颜色性状受1对或2对基因控制的结论基本一致。F_(2)分离群体Rc功能标记验证表明,Rc基因极有可能为控制‘rm257’种皮颜色的目标基因。功能标记RCGX通用性实验结果显示,云南地区地方白米品种大都存在Rc基因的缺失变异。【结论】红米种质‘rm257’的种皮颜色性状,在不同测交组合中表现为受1对显性基因控制或受2个基因位点的控制及影响。同时实验结果表明Rc基因极有可能为控制‘rm257’种皮颜色的目标基因,且云南地区多数白米地方种其种皮颜色变化都是由Rc基因缺失变异导致的。【Objective】The red rice germplasm‘rm257’showed outstanding in terms of total flavonoids and resistant starch contents in seeds.Analysis of its genetic pattern of pericarp color will lay the foundation for its practical application in breeding.At the same time,investigating the relationship between pericarp color traits with the Rc gene of red rice‘rm257’and developing corresponding functional markers will provide technical support for assisted breeding of red rice varieties in Yunnan to improve breeding efficiency.【Method】The red rice germplasm‘rm257’was used to construct test cross combinations,and F_(2) isolation population with Yunnan local white rice varieties.The genetic pattern of red rice germplasm‘rm257’was predicted by analyzing the pericarp color and separation ratio of the contemporary generation of cross test and its F_(2) segregating populations.The functional marker RCGX was developed based on the reported sequence of the Rc gene deletion mutation locus,and nine single F_(2) isolate populations were genotyped.And the genotypes were paired with corresponding individual phenotypes to determine the relationship between pericarp color and the Rc gene in red rice germplasm‘rm257’.At the same time,the versatility of functional marker RCGX was examined using 96 local species in Yunnan to evaluate the accuracy of the functional marker in determining the red rice pericarp color.【Result】Genetic pattern analysis showed that the pericarp color of red rice‘rm257’was controlled by one dominant gene pair in seven test cross combinations,and was controlled by two gene loci in two cross combinations.This was basically consistent with the previous studies reporting that the pericarp color of red rice was controlled by one or two pairs of genes.The validation of the Rc functional markers in the F_(2) isolate populations indicated that the Rc gene was most likely to be the target gene for controlling the pericarp color of‘rm257’.The results of the general experiment of functional

关 键 词：红米 种皮颜色 遗传模式 功能标记 

分 类 号：S511[农业科学—作物学]