基于便携式血糖仪的中药α-葡萄糖苷酶抑制剂筛选分析  

Screening ofα-glucosidase Inhibitor from Chinese Medicine by Portable Glucometer

作　　者：何焯斌 黄琦 沈千汇 谭国英 杨丰庆[2] 钱正明 HE Zhuobin;HUANG Qi;SHEN Qianhui;TAN Guoying;YANG Fengqing;QIAN Zhengming(Dongguan HEC Cord-yceps R&D Co.,Ltd.,Dongguan,Guangdong 523850,China;School of Chemistry and.Chemical Engineering,Chongqing University,Chongqing 401331,China)

机构地区：[1]东莞市东阳光冬虫夏草研发有限公司,广东东莞523850 [2]重庆大学化学化工学院,重庆401331

出　　处：《今日药学》2022年第2期124-127,共4页Pharmacy Today

基　　金：广东省重点领域研发计划(2020B1111110007);国家药品监督管理局药品快速检验技术重点实验室开放性课题(KF2021002)。

摘　　要：目的采用便携式血糖仪建立一种简单、有效、低成本的中药α-葡萄糖苷酶抑制剂筛选方法。方法样品溶液与0.75 U·mL^(-1)α-葡萄糖苷酶溶液、5 mg·mL^(-1)PNPG(4-硝基苯基-α-D-吡喃-葡萄糖苷)溶液37℃水浴反应2 h后,加入0.2 mol·L^(-1)碳酸钠溶液终止反应,利用便携式血糖仪进行检测,并使用传统酶标仪检测方法对结果验证。结果血糖仪与酶标仪的检测结果经t检验分析,两组数据无明显差异。结论新建立的便携式血糖仪分析方法可应用于中药α-葡萄糖苷酶抑制剂的筛选,该方法降低了筛选成本,扩大了应用范围。OBJECTIVE To establish a simple,effective and low-cost method for screeningα-glucosidase inhibitors from Chinese medicine by portable glucometer.METHODS The sample solution was reacted with 0.75 U·mL^(-1) α-glucosidase solution and 5 mg·mL^(-1) PNPG(4-nitrophenyl-α-D-glucopyranoside)solution in 37℃water bath for 2 h.After that,0.2 mol·L^(-1) sodium carbonate solution was added to stop the reaction,and then the results were tested by portable glucometer.It was verified by a microplate reader simultaneously.RESULTS The results of portable glucometer and microplate reader were no significant difference by t-test analysis.CONCLUSION The newly analysis method established by portable glucometer can be applied to screenα-glucosidase inhibitors from Chinese medicine.This method reduces the screening cost and expands the scope of application.

关 键 词：Α-葡萄糖苷酶 血糖仪 抑制剂 中药 

分 类 号：R917[医药卫生—药物分析学]