基于ADH3基因的野生大麦系统进化分析  

ADH3 gene based phylogenetic analysis of wild barley

作　　者：王梦薇 周羽 王树杰 沈辉 任喜峰[1] WANG Mengwei;ZHOU Yu;WANG Shujie;SHEN Hui;REN Xifeng(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Zhumadian Academy of Agricultural Sciences,Henan Province,Zhumadian 463000,China;Stock Breeding Farm at Xiangyang City,Hubei Province,Xiangyang 441004,China)

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]河南省驻马店市农业科学院,驻马店463000 [3]湖北省襄阳市原种繁殖场,襄阳441004

出　　处：《华中农业大学学报》2022年第3期124-130,共7页Journal of Huazhong Agricultural University

基　　金：国家大麦青稞产业技术体系(CARS-05)。

摘　　要：为探究大麦的遗传进化关系,选用91份来源于近东、中亚和我国西藏的野生大麦材料,基于乙醇脱氢酶Ⅲ基因(ADH3)对其进行系统进化分析。结果显示:近东野生大麦的单倍型个数(H=22)和遗传多样性(H_(d)=0.914,π=0.01265)均明显高于西藏野生大麦(H=5,H_(d)=0.753,π=0.01216)和中亚野生大麦(H=8,H_(d)=0.810,π=0.01110)。91份野生大麦材料主要被分成2个类群(Ⅰ和Ⅱ),81%的近东野生大麦和80%的西藏野生大麦聚类于Ⅰ类群;91%的中亚野生大麦与少部分近东和西藏野生大麦混合聚类于Ⅱ类群。同时,群体结构分析进一步佐证了西藏野生大麦与近东野生大麦之间的亲缘关系更近,与中亚野生大麦的亲缘关系较远。该研究结果明确了近东、西藏、中亚野生大麦的遗传进化关系,可为研究栽培大麦的起源提供分子依据。91 wild barley samples from the Near East,Central Asia and Tibet of China were selected and phylogenetically analyzed based on the alcohol dehydrogenaseⅢ(ADH3)gene to study the genetic evolution relationship of barley.The results showed that the number of haplotypes(H=22)and genetic diversity(H_(d)=0.914,π=0.01265)of the wild barley from Near East were significantly higher than that of the wild barley from Tibet(H=5,H_(d)=0.753,π=0.01216)and Central Asia(H=8,H_(d)=0.810,π=0.01110).Ninety-one wild barley samples were mainly divided into two groups(ⅠandⅡ).81%of wild barley from the Near East and 80% of wild barley from Tibet were clustered in groupⅠ.91%of wild barley from Central Asian and a few parts of wild barley from the Near East and Tibet were clustered in groupⅡ.The results of analyzing population structure further confirmed that the wild barley samples from Tibet were more closely related to wild barley from the Near East than to wild barley from the Central Asia.It is clarified the genetic evolution relationship of wild barley in the Near East,Tibet and Central Asia.It will provide the molecular basis for studying the origin of cultivated barley.

关 键 词：野生大麦 系统进化 群体结构 遗传多样性 种质资源 ADH3基因 

分 类 号：S512.302.4[农业科学—作物学]