组织因子微粒、内皮细胞微粒及血小板微粒在急性髓细胞白血病中检测意义  

Significance of tissue factor microparticles,endothelial cell microparticles and platelet mic⁃roparticles in acute myeloid leukemia

作　　者：戴益 许玉玲 刘莹[1] 孙娜[1] 董海群 刘振芳[1] 赵卫华[1] 程鹏[1] Dai Yi;Xu Yuling;Liu Ying;Sun Na;Dong Haiqun;Liu Zhenfang;Zhao Weihua;Cheng Peng(The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院,南宁530021

出　　处：《广西医科大学学报》2022年第5期815-819,共5页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81660025);2018年度第二批广西医药卫生自筹经费计划课题资助项目(No.Z20180908)。

摘　　要：目的:探究组织因子微粒(TF+MPs)、内皮细胞微粒(EMPs)、血小板微粒(PMPs)在急性髓细胞白血病(AML)出凝血中的检测意义。方法:将62例初治AML患者分为急性早幼粒细胞白血病(APL)组(16例)和非APL组(46例),另外选取28例健康体检者作为对照(正常组)。采用流式细胞术(FCM)检测TF+MPs、EMPs、PMPs水平。免疫浊比法检测D-二聚体(DD)和纤维蛋白降解产物(FDP)水平。结果:APL组患者TF+MPs、EMPs、PMPs、DD、FDP水平高于非APL组和正常组(均P<0.05)。DD与TF+MPs(r=0.728,P<0.01)、EMPs(r=0.790,P<0.01)、PMPs(r=0.615,P<0.01)均呈正相关关系。结论:初治AML尤其是APL患者血浆中TF+MPs、EMPs、PMPs 3种微粒表达升高,微粒水平的升高可能是引起AML凝血紊乱的原因之一。Objective:To explore the significance of tissue factor microparticles(TF+MPs),endothelial cell microparticles(EMPs)and platelet microparticles(PMPs)in bleeding and coagulation of acute myeloid leukemia(AML).Methods:A total of 62 patients with newly diagnosed AML were divided into acute promyelocytic leukemia(APL)group(n=16)and non-APL group(n=46).Another 28 healthy subjects after physical examination were selected as control group.The levels of TF+MPs,EMPs and PMPs were detected by flow cytometry(FCM).The levels of D-dimer(DD)and fibrin degradation product(FDP)were measured by immuno-turbidimetry.Results:The levels of TF+MPs,EMPs,PMPs,DD and FDP in APL group were higher than those in non-APL group and control group(P<0.05).DD was positively correlated with TF+MPs(r=0.728,P<0.01),EMPs(r=0.790,P<0.01)and PMPs(r=0.615,P<0.01).Conclusion:The expression of TF+MPs,EMPs and PMPs in plasma of patients with newly diagnosed AML,especially in patients with APL increased,which may be one of the causes of coagulation disorder in AML.

关 键 词：组织因子微粒 内皮细胞微粒 血小板微粒 急性髓细胞白血病 出凝血 

分 类 号：R733.71[医药卫生—肿瘤]