Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole(Cynoglossus semilaevis)  

作　　者：Yi-Fang Lu Qian Liu Kai-Qiang Liu Hong-Yan Wang Cheng-Hua Li Qian Wang Chang-Wei Shao 

机构地区：[1]School of Marine Sciences,Ningbo University,Ningbo,Zhejiang 315211,China [2]Key Lab of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071,China [3]Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology,Qingdao,Shandong 266000,China [4]College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China

出　　处：《Zoological Research》2022年第3期319-330,共12页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31722058,31802275,and 31472269);National Key R&D Program of China(2018YFD0900301);AoShan Talents Cultivation Program Supported by Qingdao National Laboratory for Marine Science and Technology(2017ASTCP-ES06);Taishan Scholar Project Fund of Shandong of China to C.W.S.;National Ten-Thousands Talents Special Support Program to C.W.S.;Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2020TD19);Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(20603022021018);China Agriculture Research System(CARS-47-G03);Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals,Guangdong Ocean University(KFKT2019ZD03)。

摘　　要：Chinese tongue sole(Cynoglossus semilaevis)is an economically important marine fish species with a ZZ/ZW sex determination mechanism,which can be influenced by temperature.Alternative splicing(AS)is an important mechanism regulating the expression of genes related to sex determination and gonadal differentiation,but has rarely been reported in fish.In this study,to explore the molecular regulatory mechanisms of sex determination and gonadal differentiation,we combined isoform and RNA sequencing(Iso-Seq and RNA-Seq)to perform transcriptome profiling of male and female gonads in C.semilaevis.In total,81883 and 32341 full-length transcripts were obtained in males and females,respectively.A total of 8279 AS genes were identified,including 2639 genes showing differential AS(DAS)between males and females.Many intersecting DAS genes and differentially expressed genes(DEGs)were enriched in the meiotic cell cycle pathway,and genes related to gonadal differentiation,such as esrrb and wt1a,were found to have sex-specific isoforms.Thus,this study revealed AS events in the gonadal transcriptomes of male and female C.semilaevis,described the characteristics of active transcription in the testes,and identified candidate genes for studying the regulatory mechanisms of AS during gonadal differentiation.

关 键 词：Gonadal differentiation Alternative splicing Sex-specific splicing Cynoglossus semilaevis Iso-Seq RNA-SEQ 

分 类 号：S917.4[农业科学—水产科学]