基于肠道菌群探讨复方黑骨藤有效成分组对胶原性关节炎大鼠的调节作用  

Study on the regulatory effect of active ingredient group from Periploca forrestii Schltr.compound on collagin-induced arthritis rats based on gut microbiota

作　　者：王博 张宏[1,2] 洪文静[1] 李晨阳 陈健 沈磊 李琪 WANG Bo;ZHANG Hong;HONG Wen-jing;LI Chen-yang;CHEN Jian;SHEN Lei;LI Qi(College of Life Sciences,Sichuan Normal University,Chengdu 610101,China;Plant Resources Application and Development Research Institute,Sichuan Normal University,Chengdu 610101,China)

机构地区：[1]四川师范大学生命科学学院,四川成都610101 [2]四川师范大学植物功能基因组及生物信息研究中心,四川成都610101

出　　处：《中国药理学通报》2022年第6期920-928,共9页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31240087);九寨沟地震灾区珍稀动物受损栖息地林草植被快速恢复模式研究与示范项目(No HX20200475);四川师范大学实验室创新研究项目(No SYJS2021015)。

摘　　要：目的基于肠道菌群研究复方黑骨藤有效成分组(active ingredient group from Periploca forrestii Schltr.compound,AIG)对胶原诱导性关节炎(collagin-induced arthritis,CIA)大鼠的治疗作用。方法采用牛Ⅱ型胶原乳剂制备CIA大鼠模型,采用酶联免疫方法考察各组大鼠踝关节中TNF-α、IL-6的含量变化,同时运用16s rRNA高通量测序技术测定各组大鼠肠道菌群,并通过KEGG预测菌群功能。结果AIG可明显改善CIA大鼠关节滑膜组织中炎性细胞浸润和组织增生等情况,减轻关节肿胀,以及明显降低其踝关节中IL-6和TNF-α的含量。肠道菌群结果表明AIG能增加CIA大鼠拟杆菌门、拟普雷沃菌属和Prevotella_9的相对丰度,降低杜氏杆菌属、毛螺菌属等11种菌属的相对丰度。功能预测发现AIG可能影响细胞凋亡等信号通路。结论AIG可明显改善CIA大鼠足趾肿胀,以及关节滑膜中炎性细胞浸润和组织增生,修复CIA大鼠紊乱的肠道菌群结构,可能影响其细胞凋亡通路,恢复机体免疫力。Aim To investigate the therapeutic effect of active ingredient group(AIG)in the compounds from Periploca forrestii Schltr.on collagin-induced arthritis(CIA)rats based on the variation of gut microbiota.Methods CIA model rats were induced by injection of emulsion containing bovine typeⅡcollagen,and enzyme-linked immunosorbent assay(ELISA)was used to investigate the changes of TNF-αand IL-6 in ankle joint of rats in each group.Meanwhile,the gut microbial communities were measured using 16s rRNA high-throughput sequencing technology and its function was predicted by KEGG.Results AIG could significantly restrain inflammatory cell infiltration and tissue proliferation in synovial tissues of CIA rats,and alleviate joint swelling while significantly reducing the contents of IL-6 and TNF-αin ankle joint of CIA rats.The analysis of gut microbial communities showed that AIG could increase the relative abundances of Bacteroidetes,Alloprevotella and Prevotella_9,and decrease the relative abundances of 11 genera such as Dubosiella,Lachnospiraceae_NK4A136_group,etc.in CIA rats.The functional prediction showed that AIG could influence apoptosis and other signaling pathways.Conclusions AIG can obviously alleviate joint swelling,restrain inflammatory cell infiltration and tissue proliferation in joint synovium of CIA rats,and repair the disordered gut microbiota structure of CIA rats,while affecting its apoptosis pathway and restore the body immunity.

关 键 词：复方黑骨藤 有效成分组 胶原性关节炎 肠道菌群 高通量测序 功能预测 

分 类 号：R-332[医药卫生] R284.1