三阴性乳腺癌中PD-1、PD-L1的表达与肿瘤浸润性淋巴细胞及临床病理指标的相关性  

Analysis of the correlation of the expression of PD-1 and PD-L1 with tumor infiltrating lymphocytes and clinicopathological indicators in triple negative breast cancer

作　　者：黄婉莹 杨向红[1] HUANG Wanying;YANG Xianghong(Department of Pathology,Shengjing Hospital of China Medical University,Liaoning Shenyang 110000,China)

机构地区：[1]中国医科大学附属盛京医院病理科,辽宁沈阳110000

出　　处：《现代肿瘤医学》2022年第12期2181-2185,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81773108)。

摘　　要：目的:分析三阴性乳腺癌样本中PD-1、PD-L1的表达水平及其与肿瘤浸润性淋巴细胞及临床病理指标的相关性,探讨其临床意义。方法:免疫组织化学法检测132例乳腺浸润性导管癌患者样本中PD-1、PD-L1、CD4及CD8的表达情况;苏木精-伊红染色后计数癌间质中肿瘤浸润性淋巴细胞个数;回顾性分析病理报告中各项临床病理指标并随访患者预后。分析PD-1、PD-L1在三阴组和非三阴组中表达的差异,以及其与各指标之间的相关性。结果:相较于非三阴组,三阴性乳腺癌中PD-1(P=0.034)及PD-L1(P=0.037)高表达;三阴性乳腺癌中PD-1的表达与肿瘤浸润性淋巴细胞个数(P=0.015)、CD4表达(P=0.001)、ki-67指数(P=0.012)、淋巴结转移(P=0.027)、脉管癌栓(P=0.009)呈正相关,与月经初潮时间(P=0.031)呈负相关;PD-L1的表达与肿瘤浸润性淋巴细胞个数(P=0.022)、CD4表达(P=0.004)、CD8表达(P=0.037)、ki-67指数(P=0.013)、体质量指数(P=0.004)、乳腺手术史或外伤史(P=0.029)呈正相关。结论:PD-1及PD-L1在三阴性乳腺癌中的表达水平显著高于非三阴组,其表达与肿瘤浸润性淋巴细胞的数目及分型相关,并与多种临床病理指标具有明确的相关性,提示PD-1、PD-L1及肿瘤浸润性淋巴细胞可以作为三阴性乳腺癌的诊断及预后评估的重要指标。Objective:To analyze the expression levels of PD-1 and PD-L1 in triple-negative breast cancer samples,and their correlation with tumor infiltrating lymphocytes and clinicopathological indicators,and to explore their clinical significance.Methods:Immunohistochemical method was used to detect the expression of PD-1,PD-L1,CD4 and CD8 in samples of 132 patients with invasive ductal carcinoma of breast.Tumor-infiltrating lymphocytes in the cancer stroma were counted after hematoxylin-eosin staining.The related clinicopathological indicators in the pathology report were retrospectively analyzed and the prognosis of the patients was followed up.The differences of PD-1 and PD-L1 expression in the triple-negative group and the non-triple-negative group were analyzed,as well as their correlation with each index.Results:Compared with the non-triple-negative group,PD-1(P=0.034)and PD-L1(P=0.037)were highly expressed in triple-negative breast cancer.Expression of PD-1 in triple-negative breast cancer was positively correlated with the number of tumor infiltrating lymphocytes(P=0.015),CD4 expression(P=0.001),ki-67 index(P=0.012),LN metastasis(P=0.027),vascular tumor thrombus(P=0.009),and negatively correlated with menarche time(P=0.031).PD-L1 expression was positively correlated with the number of tumor infiltrating lymphocytes(P=0.022),CD4 expression(P=0.004),CD8 expression(P=0.037),ki-67 index(P=0.013),body mass index(P=0.004),breast surgery history or trauma history(P=0.029).Conclusion:The expression levels of PD-1 and PD-L1 in triple-negative breast cancer are significantly higher than those in non-triple-negative breast cancer,and their expression is related to the number and type of tumor infiltrating lymphocytes,and has a clear relationship with a variety of clinicopathological indicators.The correlation indicates that PD-1,PD-L1 and tumor infiltrating lymphocytes can be used as important indicators for the diagnosis and prognosis evaluation of triple-negative breast cancer.

关 键 词：三阴性乳腺癌 程序性死亡受体1(PD-1) 程序性死亡受体配体1(PD-L1) 肿瘤浸润性淋巴细胞(TILs) 临床病理指标 

分 类 号：R737.9[医药卫生—肿瘤]