高危型HPV E6/E7 mRNA检测对宫颈高级别病变的预警分流价值  

The value of detecting high-risk human papillomavirus E6/E7 mRNA to triage the high grade cervical lesions in cervical cancer screening

作　　者：郭艳平[1] 王丽[1] 全诗敏 赵敏伊 杨筱凤[1] GUO Yanping;WANG Li;QUAN Shimin;ZHAO Minyi;YANG Xiaofeng(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院妇产科,陕西西安710061

出　　处：《现代肿瘤医学》2022年第12期2228-2232,共5页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:82173200)。

摘　　要：目的:通过比较检测HPV E6/E7 mRNA和HPV DNA两种HPV检测方法对宫颈高级别病变的检出能力,探讨HPV E6/E7 mRNA检测在宫颈癌筛查中的预警分流价值。方法:采集2016年7月至2017年6月就诊于我院接受宫颈癌筛查的1515例女性宫颈脱落细胞,利用转录介导扩增法检测高危型HPV E6/E7 mRNA(n=505)和PCR+膜杂交法检测HPV DNA(n=1010),以组织病理学诊断为金标准,比较两种检测方法对宫颈高级别病变检出率。结果:利用HPV E6/E7 mRNA检测组阴道镜转诊率32.69%(17/52)、HPV DNA检测组阴道镜转诊率10.62%(12/113),差异有统计学意义(P=0.001);HPV E6/E7 mRNA检测组CIN2+的检出率42.42%;HPV DNA组CIN2+的检出率28.16%,差异有统计学意义(P=0.034)。结论:与HPV DNA检测方法相比,HPV E6/E7 mRNA检测对CIN2+病变预警分流价值较高。Objective:To explore the value of detecting HPV E6/E7 mRNA in early warning triage for high grade cervical lesions in Shaan'xi province.Methods:The cervical exfoliative cells were collected from patients who accepted HPV E6/E7 mRNA test or HPV DNA test from July 2016 to June 2017 in our hospital after receiving the informed consent.Comparing the detection rate of these two methods for finding histopathological CIN2+.Results:The rate of colposcopy in the HPV E6/E7 mRNA testing group was 32.69%(17/52),and in the HPV DNA testing group was 10.62%(12/113).The difference between the two groups was significantly(P=0.001).The detection rate of CIN2+in HPV E6/E7 mRNA testing group was 42.42%,and in HPV DNA testing group was 28.16%(P=0.034).There was no relative between E6/E7 mRNA transcription levels and the grading cervical lesions.Conclusion:HPV E6/E7 mRNA test has higher value than HPV DNA test for early warning triage for high grade cervical lesions in cervical cancer screening.

关 键 词：HPV E6/E7 mRNA HPV DNA 宫颈病变 筛查 转录水平 

分 类 号：R737.33[医药卫生—肿瘤]