3株禽副黏病毒4型国内分离株的基因组序列及生物学特性分析  

Analysis on the Genome Sequence and Biological Characteristics of Three Avian Paramyxovirus-4 Isolated in China

作　　者：李峥 舒波 彭程[1] 于晓慧 李金平[1] 侯广宇[1] 蒋文明[1] 王静静[1] 王桂军[2] 刘华雷[1] Li Zheng;Shu Bo;Peng Cheng;Yu Xiaohui;Li Jinping;Hou Guangyu;Jiang Wenming;Wang Jingjing;Wang Guijun;Liu Hualei(China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China;Anhui Agricultural University College of Animal Science and Technology,Hefei,AnHui 230000,China;Jiangxi Agricultural University College of Animal Science and Technology,Nanchang,Jiangxi 330000,China)

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]安徽农业大学动物科技学院,安徽合肥230000 [3]江西农业大学动物科技学院,江西南昌330000

出　　处：《中国动物检疫》2022年第6期30-36,共7页China Animal Health Inspection

摘　　要：为了解我国近年来新出现的禽副黏病毒4型(APMV-4)的生物学特性,对2020年分离鉴定的3株鸭源APMV-4进行致病性分析和基因组测定。结果显示:3株APMV-4分离株F蛋白裂解位点均为116DIPQR↓F121,1日龄雏鸡脑内接种致病指数(ICPI)为0~0.23,对鸡表现为低致病力。3株分离毒株基因组长度均为15054 nt,基因组结构顺序均为3'-N-P-M-F-HN-L-5',符合典型的副黏病毒基因组“六碱基原则”;病毒3'端具有55 nt的前导序列,5'端存在17 nt的尾随序列,各基因间有9~42 nt的基因间隔序列;3株分离毒株与APMV-4代表株同源性最高(97.2%~97.5%),与其余禽副黏病毒同源性为37.1%~47.3%,与中国香港、韩国分离株高度同源。结果表明,我国新出现的鸭源APMV-4为弱毒株,基因组序列同源性较高,关键氨基酸位点未发生变异。本研究为进一步了解APMV-4和科学防控APMV-4感染提供了参考。In order to investigate the biological characteristics of avian paramyxovirus type 4(APMV-4)newly emerged in China in recent years,pathogenicity and genome analysis were carried out on three APMV-4 strains isolated from ducks in 2020.The results showed that all the F protein cleavage sites of the three strains was 116DIPQR↓F121.The intracerebral pathogenicity index(ICPI)in one-day-old chickens was from 0 to 0.23,indicating low pathogenicity to chickens.The genome length of the three strains was 15054 nt,and 3'-N-P-M-F-HN-L-5'in the order of structure,which conformed to the typical“six bases principle”of paramyxovirus genome;55 nt leading sequence was observed at 3'end of the virus,and 17 nt trailing sequence at 5'end,a gene interval sequence of 9 to 42 nt was available between each gene;the homology of the three strains with the representative strain of APMV-4 was highest(97.2%to 97.5%),37.1%to 47.3%with other APMVs,and highly closest to those isolated in Hong Kong China and Korea.In conclusion,APMV-4 was low virulent as a new duck derived strain in China,with high homology and no mutation was observed in key amino acid sites.A reference was thus provided for further research on APMV-4 and scientific prevention and control of its infection.

关 键 词：禽副黏病毒4型 野鸭 基因组序列 生物学特性 

分 类 号：S852.65[农业科学—基础兽医学]