肝纤维化动物实验模型的研究进展  

Research progress of animal experimental model of hepatic fibrosis

作　　者：南洋[1] 董辉[1] 王志刚[1] 刘树民[2] NAN Yang;DONG Hui;WANG Zhigang;LIU Shumin(College of Pharmacy,Heilongjiang University of Chinese Medicine,Heilongjiang Province,Harbin150040,China;Chinese Medicine Research Institute,Heilongjiang University of Chinese Medicine,Heilongjiang Province,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040

出　　处：《中国医药导报》2022年第12期34-37,共4页China Medical Herald

基　　金：国家自然科学基金面上项目(81573592);国家自然科学基金青年科学基金面上项目(81102896);黑龙江省自然科学基金联合引导项目(LH2019H056);中国博士后科学基金项目(2017M621319)。

摘　　要：肝纤维化的致病因素在于创伤愈合反应的渐进过程,肝纤维化可进展为肝硬化,并进一步发展为肝癌。目前科研人员在肝纤维化研究方面付出了相当大的努力,希望通过阐明这种疾病的发病机制,建立有效的诊断和治疗方案。本文目的是从动物实验模型建立的理论基础、实验动物的选择、操作方法、试剂的用量、注意事项和模型的优缺点及适用方向进行详细分析,希望可以借此帮助科研人员根据不同的实验需求,在研究肝纤维化时做出更好的选择。肝纤维化的动物实验模型需要注意在建模时的随行观察,以免给药过度形成更为严重的疾病模型如肝硬化模型等。肝纤维化的动物实验方法较多,有化学方法、饮食法、手术法、感染法和遗传修饰小鼠模型,通过对文献内容的比较和分析,发现四氯化碳法是比较普遍适用的方法,具有方法简单、可靠性强、价格低廉等优点。The pathogenesis of hepatic fibrosis is the progressive process of wound healing reaction,hepatic fibrosis can progress to cirrhosis,and further development of liver cancer.At present,researchers have paid considerable efforts in the research of hepatic fibrosis,hoping to clarify the pathogenesis of this disease and establish effective diagnosis and treatment plan.The purpose of this article is from the animal experimental model of theoretical basis,the selection of experimental animal,the operation method,the usage of reagents,the matters needing attention,and the advantages and disadvantages of the model,and its application,hoping to help in accordance with the different requirements of the scientific research personnel and make better choices in the study of liver fibrosis.The animal experimental model of hepatic fibrosis needs to be observed during the modeling to avoid excessive administration of drugs to form a more serious disease model such as cirrhosis.There are many animal experimental methods for hepatic fibrosis,including chemical methods,dietary methods,surgical methods,infection methods,and genetically modified mouse models.Through comparison and analysis of literature contents,it is found that carbon tetrachloride method is generally applicable and has the advantages of simple method,high reliability,and low price.

关 键 词：肝纤维化 动物模型 星状细胞 四氯化碳 

分 类 号：R-332[医药卫生]