Forthrightly monitoring ferroptosis induced by endoplasmic reticulum stresses through fluorescence lifetime imaging of microviscosity increases with a specific rotor  

作　　者：Chuanhao Liu Lin Zhou Lijuan Xie Ying Zheng Huizi Man Yi Xiao 

机构地区：[1]Engineering Research Center of Molecular Medicine of Ministry of Education,Key Laboratory of Fujian Molecular Medicine,School of Medicine,Huaqiao University,Quanzhou 362021,China [2]State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian 116024,China

出　　处：《Chinese Chemical Letters》2022年第5期2537-2540,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21776037,22174009);Dalian Sci-ence and Technology Innovation Fund(No.2020JJ25CY014);Quanzhou Science and Technology Plan Project(No.2019C033R).

摘　　要：To test the hypothesis that the microviscosity changes of Endoplasmic Reticulum(ER)can be a useful indicator of ferroptosis promoted by ER Stresses(ERS),a new ER targeting viscosity rotor,L-Vis-1 was developed and applied in the quantitation of viscosity by FLIM imaging in live cells.The FLIM imag-ing exhibited an excellent resolution almost as good as the corresponding confocal imaging,more sig-nificantly,during ferroptosis processes promoted by different types of ERS,the viscosity increases were clearly monitored by FLIM of L-Vis-1 within ER,which has not been demonstrated before.

关 键 词：Ferroptosis VISCOSITY FLIM ERS ROTOR 

分 类 号：Q2-33[生物学—细胞生物学] O657.3[理学—分析化学]