基于SSR技术的21个泡核桃品种遗传多样性和亲缘分析  

Genetic Diversity and Relationship Analysis of 21 Walnut Varieties based on SSR Markers

作　　者：徐令文 王毅 原晓龙 陆斌 郝佳波 张雨 马骏 王飞 Xu Lingwen;Wang Yi;Yuan Xiaolong;Lu Bin;Hao Jiabo;Zhang Yu;Ma Jun;Wang Fei(College of Forestry,Southwest Forestry University,Kunming,650224;Key Laboratory of Forest Plant Cultivation and Utilization of Yunnan Province,Yunnan Laboratory for Conservation of Rare,Endangered&Endemic Forest Plants,State Forestry Administration,Yunnan Academy of Forestry and Grassland,Kunming,650201;Kunming Forestry Technology Promotion Center,Kunming,650033)

机构地区：[1]西南林业大学林学院,昆明650224 [2]云南省林业和草原科学院云南省森林植物培育与开发利用重点实验室国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,昆明650201 [3]昆明市林业技术推广总站,昆明650033

出　　处：《分子植物育种》2022年第7期2359-2365,共7页Molecular Plant Breeding

基　　金：由泡核桃种质资源调查收集(2019FY100801_02_02);云南省林业科学院核桃产业技术省创新团队(2018HC015);分子生物学研究团队(LKYTD-2020-001);核桃产业国家创新联盟(NAWI)共同资助;。

摘　　要：为了解云南省核桃的种质资源信息,本研究以21个泡核桃品种资源为试验材料,使用SSR技术进行遗传多样性分析和聚类分析。结果表明,在21个泡核桃品种样本中使用的12对引物平均等位基因数目为7.9167,平均每个位点有效等位基因数目、香农指数(Shannon wiener index)、多态信息含量(Polymorphism information content)分别为4.3516、1.6436、0.7055。12对引物多态信息含量(PIC)高于0.25,说明这12对引物对样本均有良好多态性。亲缘关系分析中,21个泡核桃品种被分为7个类群,说明这21个泡核桃品种遗传多样性较为丰富,在品种选育过程中基因交流广泛,遗传背景较为复杂。In order to grasp the germplasm resources of walnut in Yunnan Province,the genetic diversity and relationship of 21 walnuts varieties commonly planted in Yunnan Province were analyzed using SSR fluorescent labeling technology.The results showed that the average number of allelic loci of 12 pairs of primers used in 21 walnut species samples was 7.9167.The mean number of alleles,Shannon index(I)and polymorphic information content(PIC)per locus were 4.3516,1.6436 and 0.7055.Twelve pairs of polymorphism information content(PIC)were higher than 0.25,indicating that the 12 pairs of primers had good polymorphism in the samples.In the phylogenetic analysis,the 21 walnut varieties were divided into 7 subgroups,indicating that the 21 walnut varieties had rich genetic diversity,extensive gene exchange and complex genetic background in the breeding process.

关 键 词：核桃 SSR 遗传学分析 聚类分析 

分 类 号：S664.1[农业科学—果树学]