应用SSR分子标记揭示马铃薯不同脱毒株系间的遗传差异  

Genetic Differences Among Different Virus-free Potato Lines were Revealed by SSR Molecular markers

作　　者：杜坚 周平 徐笑宇 郭华春[1] 王琼[1] Du Jian;Zhou Ping;Xu Xiaoyu;Guo Huachun;Wang Qiong(Tuber and Root Crops Research Institute,College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,650201)

机构地区：[1]云南农业大学农学与生物技术学院薯类作物研究所,昆明650201

出　　处：《分子植物育种》2022年第8期2665-2673,共9页Molecular Plant Breeding

基　　金：国家现代农业产业技术体系项目(CARS-09-15P);云南科技项目(202102AE090018)共同资助。

摘　　要：为分析马铃薯茎尖脱毒处理过程是否会引起不同脱毒株系间的遗传结构发生改变,本研究利用经过筛选出来的10对SSR引物对3份马铃薯品系(‘滇薯1418’,‘19(2)-5D1’,‘2012-20-5D1’)母株及11份相应的脱毒株系开展了SSR分析。聚类分析结果表明,所有参试的试验材料可被聚为三大类,第一类包括‘滇薯1418’母株系及其5个脱毒株系(1-1,1-2,1-4,1-9,1-10),与母株‘滇薯1418’遗传距离最相近的是1-10;第二类包括‘19(2)-5D1’母株系及其三个脱毒株系(4-2,4-4,4-5),与母株‘19(2)-5D1’遗传距离最相近的是4-5;第三类包括‘2012-20-5D1’母株系及其3个脱毒株系(8-3,8-4,8-12),与母株‘2012-20-5D1’遗传距离最相近的是8-3。脱毒处理后,不同脱毒株系间试管薯诱导能力亦存在一定差异,但总体仍然保持有较为稳定的试管微型薯生产能力,且试管薯薯型一致。选择一个与母株遗传距离最近的株系来代表母株系扩繁,在保证脱去病毒的基础上,亦可最大限度保持原品种的遗传特性。本研究为马铃薯种质资源的有效利用及科学选用脱毒株系提供依据。Investigation on the possible variation of the genetic structure among parent potato lines and the corresponding virus-free materials has been conducted.A total of 10 pairs of SSR primers were selected to carry out the genetic diversity analysis study on three parent potato lines known as the’Dianshu1418’,’19(2)-5D1’,’2012-20-5D1’and the 11 virus-free lines derived from them.Cluster analysis showed that all the experimental materials could be grouped into three categories.The first category included the parent plant’Dianshu1418’and the five virus-free lines derived from(1-1,1-2,1-4,1-9,1-10),within which the virus-free line 1-10 was intimately related to’Dianshu1418’genetically.The second group consisted of the maternal plant’19(2)-5D1’and three correlated virus-free lines(4-2,4-4,4-5),and line 4-5 had the closest genetic distance to’19(2)-5D1’.Likewise,the third class was comprised of’2012-20-5D1’and the three derived virus-free lines(8-3,8-4,8-12),it was revealed that8-3 was in close proximity to the parental line.Some differences in tuber induction of the virus-free lines were reflected after the virus-free treatment,however not significant in the overall tuber productivity,while the morphology among test tube tubers remained consistent.It could be therefore concluded that the propagative capability and genetic characteristics,as well as the removal of virus infection of the primitive potato materials might be effectively retained through using the virus-free lines harboring the least genetic discrepancy to the parental potato plants.The study has provided scientific support for the reasonable application of potato germplasms,as well as the selection of virus-free potato lines for future development purposes.

关 键 词：马铃薯 脱毒株系 SSR分子标记 遗传差异 

分 类 号：S532[农业科学—作物学]