化学蛋白质组学方法解析天然产物生物合成途径  

Elucidating the Biosynthetic Pathways of Natural Products by Chemical Proteomics

作　　者：李晴 沈陈金鑫 薛付冲 李贤慧 郑豪 耿倚云 许静远 邓张双 周怡青 LI Qing;SHEN Chenjinxin;XUE Fuchong;LI Xianhui;ZHENG Hao;GENG Yiyun;XU Jingyuan;DENG Zhangshuang;ZHOU Yiqing(School of Biotechnology&Food Engineering,Changshu Institute of Technology,Suzhou 215500,China;College of Biological&Pharmaceutical Science,Key Laboratory of Functional Yeast of China National Light Industry,China Three Gorges Univ.,Yichang 443002,China)

机构地区：[1]常熟理工学院生物与食品工程学院,江苏苏州215500 [2]中国轻工业功能酵母重点实验室(三峡大学生物与制药学院),湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2022年第4期107-112,共6页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金项目(22077011,82003885)。

摘　　要：天然产物因其结构新颖性、生物相容性和功能多样性成为现代药物研发创新的重要源泉.传统植物来源的天然产物生物合成途径研究主要基于基因组学和生物信息学联合分析和生物学功能验证,具有一定局限性.基于小分子探针的化学蛋白质组学技术的飞速发展为植物天然产物的生物合成途径解析提供了新的机遇.本文以胆固醇、原花青素、桑白皮中Deils-Adel类型天然产物和甜菊糖苷等天然产物为例,简要概括了近10年来分子探针和化学蛋白质组学在天然产物生物合成途径解析中的应用.Natural products have become an important source of innovative drugs due to their structural novelty,biocompatibility,and functional diversity.Conventionally,the studies of biosynthetic pathways of plant natural products rely on genomic mining and bioinformatics analysis,followed by functional validation via biochemical methods.Nevertheless,the approaches have certain limitations and it is more difficult to be applied in the study of natural products of eukaryotes.The rapid development of chemical proteomics provides new opportunities for the study of biosynthetic pathways of natural products from plants.The application of activity/affinity-based probes-based chemoproteomic strategy in the studying of biosynthetic pathway of eukaryotic natural products is summarized in this review,by using cholesterols,proanthocyanidin,Diels-Alder type natural products from Morusalba,and stevioside as representative examples,the function and structure of key enzymes in the biosynthesis pathway of several natural products.

关 键 词：天然产物 化学蛋白组学 生物合成 分子探针 

分 类 号：TQ93[化学工程] R914[医药卫生—药物化学]