微生物法测定功能性饮料中维生素B_(12)含量研究  

Study on determination of vitamin B_(12) in functional beverage by microbiological method

作　　者：晏涛 刘萍 郦娟 陈琼 陈哲 郑楠 曾慧君 YAN Tao;LIU Ping;LI Juan;CHEN Qiong;CHEN Zhe;ZHENG Nan;ZENG Huijun(Wuhan Institute for Food and Cosmetic Control, Wuhan, 430040, China;Key Laboratory of Edible Oil Quality and Safety for State Market Regulation, Wuhan 430072, China;Wuhan Polytechnic, Wuhan 430072, China)

机构地区：[1]武汉食品化妆品检验所,武汉430040 [2]国家市场监管重点实验室(食用油质量与安全),武汉430040 [3]武汉职业技术学院,武汉430072

出　　处：《华中师范大学学报（自然科学版）》2022年第3期476-480,486,共6页Journal of Central China Normal University：Natural Sciences

基　　金：国家市场监督管理总局科技计划项目(2020MK131);湖北省教育厅科学技术研究项目(B2018456).

摘　　要：该文建立了测定功能性饮料中维生素B_(12)含量的微生物法.参考食品安全国家标准GB 5413.14—2010婴幼儿食品和乳品中维生素B_(12)的测定方法,利用莱士曼氏乳酸杆菌生长速率与B_(12)浓度的线性关系,制备标准曲线,根据标准曲线计算样品中B_(12)的含量.根据功能性饮料与奶粉基底不同,对国标法中样品处理进行优化改良,同时实验中进行了加标回收对实验过程进行质量控制,并使用试剂盒法对测定结果进行比对.该方法操作简便,灵敏性及准确性高,可适用于功能性饮料中维生素B_(12)含量的检测.The determination of vitamin B_(12) is one of the most challenging problems in food analysis worldwide.A new method for detecting vitamin B_(12) in functional beverages was established.With reference to the determination method of vitamin B_(12) in infant foods and dairy products in the national food safety standard GB 5413.14-2010,which stems from the linear relationship between the growth rate of Lactobacillus leishmanii and B_(12) concentration,the standard curve was constructed and the content of vitamin B_(12) in samples were measured.Due to the differences among the substrates of functional beverages and milk powder,sample treatment methods were optimized based on the national standard.In addition,the quality control by standard additional recovery was conducted and the test result was compared with commercial assay kit.These results indicated that this method is able to be applied to the determination of vitamin B_(12) in functional beverages because of its simplicity,sensitivity and accuracy.

关 键 词：维生素B_(12) 功能性饮料 微生物法 检测 

分 类 号：R155.59[医药卫生—营养与食品卫生学]